4tnh
RT XFEL structure of Photosystem II in the dark state at 4.9 A resolutionRT XFEL structure of Photosystem II in the dark state at 4.9 A resolution
Structural highlights
FunctionPSBE_THEVB This b-type cytochrome is tightly associated with the reaction center of photosystem II (PSII). PSII is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation.[HAMAP-Rule:MF_00642][1] [2] [3] Publication Abstract from PubMedThe dioxygen we breathe is formed by light-induced oxidation of water in photosystem II. O2 formation takes place at a catalytic manganese cluster within milliseconds after the photosystem II reaction centre is excited by three single-turnover flashes. Here we present combined X-ray emission spectra and diffraction data of 2-flash (2F) and 3-flash (3F) photosystem II samples, and of a transient 3F' state (250 mus after the third flash), collected under functional conditions using an X-ray free electron laser. The spectra show that the initial O-O bond formation, coupled to Mn reduction, does not yet occur within 250 mus after the third flash. Diffraction data of all states studied exhibit an anomalous scattering signal from Mn but show no significant structural changes at the present resolution of 4.5 A. This study represents the initial frames in a molecular movie of the structural changes during the catalytic reaction in photosystem II. Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy.,Kern J, Tran R, Alonso-Mori R, Koroidov S, Echols N, Hattne J, Ibrahim M, Gul S, Laksmono H, Sierra RG, Gildea RJ, Han G, Hellmich J, Lassalle-Kaiser B, Chatterjee R, Brewster AS, Stan CA, Glockner C, Lampe A, DiFiore D, Milathianaki D, Fry AR, Seibert MM, Koglin JE, Gallo E, Uhlig J, Sokaras D, Weng TC, Zwart PH, Skinner DE, Bogan MJ, Messerschmidt M, Glatzel P, Williams GJ, Boutet S, Adams PD, Zouni A, Messinger J, Sauter NK, Bergmann U, Yano J, Yachandra VK Nat Commun. 2014 Jul 9;5:4371. doi: 10.1038/ncomms5371. PMID:25006873[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Large Structures
- Thermosynechococcus vestitus BP-1
- Adams PD
- Alonso-Mori R
- Bergmann U
- Bogan MJ
- Boutet S
- Brewster A
- Chatterjee R
- DiFiore D
- Echols N
- Fry AR
- Gallo E
- Gildea RJ
- Glatzel P
- Gloeckner C
- Gul S
- Han G
- Hattne J
- Hellmich J
- Ibrahim M
- Kern J
- Koglin JE
- Koroidov S
- Laksmono H
- Lampe A
- Lassalle-Kaiser B
- Messerschmidt M
- Messinger J
- Milathianaki D
- Sauter NK
- Seibert MM
- Sierra RG
- Skinner DE
- Sokaras D
- Stan CA
- Tran R
- Uhlig J
- Weng T-C
- Williams GJ
- Yachandra VK
- Yano J
- Zouni A
- Zwart PH