Transcriptional activator: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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-<StructureSection load='1h89' size='350' side='right' caption='Structure of transcriptional activator C-Myb DNA-binding domain (pink) complex with DNA, CAAT/enhancer binding protein β (grey and green) and K+ ion (purple) (PDB entry [[1h89]])' scene=''>
+<StructureSection load='1h89' size='350' side='right' caption='Structure of transcriptional activator C-Myb DNA-binding domain (deeppink) complex with DNA, CAAT/enhancer binding protein β (cyan and green) and K+ ion (purple) (PDB entry [[1h89]])' scene='55/553105/Cv/1'>
+__TOC__
-'''Transcriptional activators''' (TA) are proteins which bind to DNA and stimulate nearby gene transcription.  TA transcription stimulation is achieved by enhancing RNA polymerase binding or by enhancing the formation of open complex which is required for initiation of transcription.  TA bind to a specific DNA sequence in or near an operon via their DNA-binding domain (DBD).  TA contains 3 domains: DBD, trans-activating domain (TAD) which binds coregulator proteins and an optional ligand-binding domain (LBD) which sense external signals.
+== Function ==
+'''Transcriptional activators''' or '''Transcriptional regulators''' (TA) are proteins which bind to DNA and stimulate nearby gene transcription.  TA transcription stimulation is achieved by enhancing RNA polymerase binding or by enhancing the formation of open complex which is required for initiation of transcription.  TA bind to a specific DNA sequence in or near an operon via their DNA-binding domain (DBD)<ref>PMID:16464826</ref>. '''PrfA''' is an activator of virulence genes in ''Listeria monocytogenes''<ref>PMID:10564496</ref>. See also [[Listeriolysin positive regulatory factor A]].
+== Structural highlights ==
+TA contains 3 domains: DBD, trans-activating domain (TAD) which binds coregulator proteins and an optional ligand-binding domain (LBD) which senses external signals.
+*<scene name='55/553105/Cv/5'>Transcriptional activator C-Myb DNA-binding domain complex with DNA, CAAT/enhancer binding protein β and K+ ion </scene> (PDB entry [[1h89]]).
+*<scene name='55/553105/Cv/6'>1st K soordination site</scene>.
+*<scene name='55/553105/Cv/7'>2nd K soordination site</scene>. Water molecules are shown as red spheres.
 == 3D Structures of transcriptional activator==
+[[Transcriptional activator 3D structures]]
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+</StructureSection>
-===Gcn4 TA===
-See [[Gcn4]]
-===Gcn5 TA===
-[[1e6i]] – yTA brododomain + histone H4 peptide - yeast<br />
-[[1ygh]] – yTA histone acetyltransferase domain <br />
-===C-Myb  TA===
-[[1mse]], [[1msf]] – TA DBD + DNA - NMR<br />
-[[1mbe]], [[1mbf]] – mTA DBD repeat 1 + DNA – mouse - NMR<br />
-[[1mbg]], [[1mbh]] – mTA DBD repeat 2 + DNA - NMR<br />
-[[1mbj]], [[1mbk]] – mTA DBD repeat 3 + DNA - NMR<br />
-[[1h88]] – mTA DBD + DNA + CCAAT/enhancer binding protein β<br />
-[[1h89]] – mTA DBD + DNA + CAAT/enhancer binding protein β<br />
-[[1guu]] – mTA DBD repeat 1  <br />
-[[1gv2]] – mTA DBD repeat 2,3 <br />
-[[1gv5]] – mTA DBD repeat 2  <br />
-[[1gvd]] – mTA DBD repeat 2 (mutant) <br />
-[[1idy]], [[1idz]] – mTA DBD repeat 3 (mutant) - NMR<br />
-[[1sb0]], [[2agh]] – mTA TAD + CREB-binding protein - NMR<br />
-===Psp operon  TA===
-[[2bjw]] – EcTA AAA domain – ''Escherichia coli''<br />
-[[2bjv]], [[2c9c]] – EcTA AAA domain (mutant)<br />
-[[2c96]] – EcTA AAA domain + ATP<br />
-[[2c98]] – EcTA AAA domain + ADP<br />
-[[2c99]] – EcTA AAA domain + AMPPNP<br />
-[[2vii]] – EcTA AAA domain + AMP + Mg<br />
-===TenA  TA===
-[[1tyh]], [[1yaf]] – BsTA – ''Bacillus subtilis''<br />
-[[2qzc]] – TA – ''Sulfolobus solfataricus''<br />
-[[3no6]] – TA – ''Staphylococcus epidermidis''<br />
-[[1yak]] – BsTA + pyrimidine derivative<br />
-[[2qcx]] – BsTA (mutant) + pyrimidine derivative<br />
-===LasR  TA===
-[[2uv0]] – PaTA LBD – ''Pseudomonas aeruginosa''<br />
-[[3ix3]] – PaTA LBD + oxo-dodecanoyl-homoserine lactone<br />
-[[3ix4]], [[3ix8]], [[3jpu]] – PaTA LBD + benzoate derivative<br />
-[[4ng2]] – PaTA LBD + protein<br />
-===CadC  TA===
-[[3ly7]], [[3lya]] – EcTA periplasmic domain <br />
-[[3ly8]], [[3ly9]] – EcTA periplasmic domain (mutant)<br />
-===CynR  TA===
-[[2hxr]] – EcTA LBD <br />
-[[3hfu]] – EcTA LBD + N3<br />
-===Mal-Rpel  TA===
-[[2v51]], [[2v52]] – mTA residues 16-41 + actin <br />
-[[2yje]], [[2yjf]] – mTA Rpel domain + actin <br />
-===Brg1  TA===
-[[3uvd]] – TA bromodomain + pyrrolidone derivative - human<br />
-===VP30  TA===
-[[2i8b]] – TA C terminal – Zaire ebolavirus<br />
-[[3v7o]] – TA C terminal – Reston ebolavirus<br />
-===TraR  TA===
-[[1l3l]] – AtTA + pheromone + DNA – ''Agrobacterium tumefaciens'' <br />
-[[1h0m]] – AtTA + auto-inducer + DNA  <br />
-===Flagelar  TA===
-[[1g8e]] – EcTA  FLHD<br />
-[[2avu]] – EcTA  FLHC+FLHD<br />
-===TipA  TA===
-[[1ny9]] – SlTA  antibiotic binding domain – ''Streptomyces lividans'' - NMR<br />
-[[2vz4]] – SlTA DBD + DNA<br />
-===MtaN  TA===
-[[1r8d]] – BsTA  <br />
-===RfaH  TA===
-[[2oug]] – EcTA  <br />
-[[2lcl]] – EcTA C terminal - NMR<br />
-===SoxR  TA===
-[[2zhh]] – EcTA  <br />
-[[2zhg]] – EcTA  + DNA<br />
-===AmpR  TA===
-[[3kos]] – CfTA  effector binding domain – ''Citrobacter freundii''<br />
-[[3kot]] – CfTA  effector binding domain (mutant)<br />
-===GlxR  TA===
-[[3r6s]] – TA +cAMP – ''Corynebacterium glutamicum'' <br />
-===OxyR  TA===
-[[3t22]] – TA regulatory domain – ''Porphyromonas gingivalis'' <br />
-===PlcR  TA===
-[[4fsc]] – BtTA - ''Bacillus thuringiensis'' <br />
-[[3u3w]] – BtTA + DNA + PAPR peptide  <br />
-===VirB  TA===
-[[3vwb]], [[3w2a]], [[3w3c]] – TA  core domain + DNA – ''Shigella flexneri''<br />
-===MafA  TA===
-[[4eot]] – hTA  DBD<br />
-===CysB TA===
-[[4gwo]], [[4gxa]] – TA C terminal – ''Salmonella typhimurium''<br />
-===HlyU  TA===
-[[4k2e]] – TA  - ''Vibrio cholerae''<br />
-===Aft2  TA===
-[[4lmg]] – yTA + DNA<br />
+== References ==
+<references/>
 [[Category:Topic Page]]