Haloperoxidase: Difference between revisions

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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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-{{STRUCTURE_1qi9|  PDB=1qi9  | SIZE=400| SCENE= |right|CAPTION=Bromoperoxidase complex with VO4 and Br- (purple) ions,  [[1qi9]] }}
+<StructureSection load='1qi9' size='400' side='right' scene='48/486480/Cv/1' caption='Bromoperoxidase complex with VO4 and I- (purple) ions,  [[1qi9]]'>
 == Function ==
-'''Haloperoxidases''' catalyze the oxidation of halides by hydrogen peroxide while adding a halide to hydrocarbons.  They are classified as '''chloroperoxldase''' (CPO), '''bromoperoxidase''' (BPO) and '''iodoperoxidase''' (IPO) according to the halide which they oxidize.  CPO is heme-containing, vanadium-containing or metal-free.  BPO from marine algae is vanadium-containing<ref>PMID:19363038</ref>.
+'''Haloperoxidases''' catalyze the oxidation of halides by hydrogen peroxide while adding a halide to hydrocarbons.  They are classified as '''chloroperoxldase''' (CPO), '''bromoperoxidase''' (BPO) and '''iodoperoxidase''' (IPO) according to the halide which they oxidize.
+*'''CPO''' is heme-containing, vanadium-containing or metal-free.
+*'''BPO''' is from marine algae is vanadium-containing<ref>PMID:19363038</ref>.
 == Structural highlights ==
-The vanadate io shows a trigonal bipyramidal coordination<ref>PMID:10543953</ref>.
+The <scene name='48/486480/Cv/5'>vanadate ion shows a trigonal bipyramidal coordination</scene>.  The iodine atoms are coordinated to tyrosine residues: <scene name='48/486480/Cv/6'>first coordination site</scene> and <scene name='48/486480/Cv/7'>second coordination site</scene> <ref>PMID:10543953</ref>. Water molecules shown as red spheres.
+</StructureSection>
 ==3D structures of haloperoxidase==
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 {{#tree:id=OrganizedByTopic|openlevels=0|
-*Vanadium-containing chloroperoxidase
+*Vanadium-dependent chloroperoxidase
-**[[1vnc]], [[1vni]], [[1idq]], [[1idu]] – CiCPO – ''Curvularia inaequalis''<br />
+**[[1vnc]], [[1vni]], [[1idq]], [[1idu]] – CiCPO + VO4 – ''Curvularia inaequalis''<br />
-**[[1vne]], [[1vnf]], [[1vng]], [[1vnh]] – CiCPO (mutant)<br />
+**[[1vne]], [[1vnf]], [[1vng]], [[1vnh]] – CiCPO (mutant) + VO4<br />
-**[[1vns]] – CiCPO no vanadium
+**[[1vns]], [[3bb0]] – CiCPO <br />
+**[[3w35]] – StCPO – ''Streptomyces''<br />
+**[[3w36]] – StCPO + VO4<br />
+**[[5lpc]] – CPO – ''Acaryochloris marina''<br />
 *Heme-containing chloroperoxidase
 **[[1cpo]], [[2cpo]] – LfCPO – ''Leptoxyphium fumago''<br />
-**[[2civ]] – LfCPO + Br<br />
+**[[2civ]], [[2j18]], [[2j19]] – LfCPO + Br<br />
 **[[2ciw]] – LfCPO + I<br />
 **[[2cix]] – LfCPO + cyclopentane-dione + Br<br />
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 **[[1a8s]] – PfCPO F + propanoic acid – ''Pseudomonas fluorescens''<br />
 **[[1a8u]] – PfCPO T + benzoic acid<br />
-**[[1a88]] - CPO L – ''Streptomyces lividans''
+**[[1a88]] - CPO L – ''Streptomyces lividans''<br />
+**[[4dgq]] – CPO – ''Burkholderia cenocepacia''<br />
 *Metal-free bromoperoxidase
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 **[[1a8q]] – SaBPO A1<br />
 **[[1bro]] - SaBPO A2<br />
-**[[1brt]] – SaBPO A2 (mutant)<br />
+**[[1brt]], [[4iq4]], [[4itv]], [[4ivj]], [[4qes]], [[4qf0]], [[4qff]] – SaBPO A2 (mutant)<br />
 **[[3fob]] – BPO – ''Bacillus anthracis''
 *Vanadium-containing bromoperoxidase
-**[[1qi9]] – BPO + I – ''Ascophyllum nodosum''<br />
+**[[1qi9]] – AnBPO + I + VO4 – ''Ascophyllum nodosum''<br />
-**[[1up8]], [[1qhb]] – BPO no vanadium – ''Corallina pilulifera''
+**[[5aa6]] – AnBPO-2 + VO4<br />
+**[[1up8]], [[1qhb]], [[7qyy]] – CpBPO – ''Corallina pilulifera''<br />
+**[[7qvw]] – CpBPO (mutant) <br />
+**[[7qwi]] – CpBPO + VO4<br />
+**[[7qw3]] – CpBPO (mutant) + Br<br />
 *Vanadium-containing iodoperoxidase