Luciferase: Difference between revisions

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[[Image:3iep.png|left|200px|thumb|Crystal Structure of Luciferase [[3iep]]]]
<StructureSection load='3iep' size='350' side='right' scene='Luciferase/Cv/1' caption='Luciferase (PDB code [[3iep]])'>
{{STRUCTURE_3iep|  PDB=3iep  | SIZE=400| SCENE=Luciferase/Cv/1 |right|CAPTION=Luciferase [[3iep]] }}
== Function ==
[[Luciferase]] (Luc) or '''luciferin monooxygenase''' (Lucmo)is an oxidative enzyme used in bioluminescence.  It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light<ref>PMID:2030669</ref>.  See detailed analysis in<br />
* [[Luciola cruciata luciferase]]<br />
* [[Luciferase FMN complex- Vibrio harveyi]]<br />
* [[Dinoflagellate luciferase]]<br />
* [[Luciferase (Hebrew)]].


See [[Colored & Bioluminescent Protein]].


== Relevance ==
Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>.  '''NanoLuc luciferase''' is a small mutated luciferase from shrimp with enhanced stability and a significant increase in luminescence <ref>PMID:27045664</ref>.


[[Luciferase]] (Luc) is an oxidative enzyme used in bioluminescence.  It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light. The images at the left and at the right correspond to one representative Luc, ''i.e.'' the crystal structure of firefly Luciferase ([[3iep]]).  See detailed analysis of [[Luciola cruciata luciferase]] and [[Luciferase FMN complex- Vibrio harveyi]].
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== 3D Structures of Luciferase ==
== 3D Structures of Luciferase ==
[[Luciferase 3D structures]]


''Update June 2012''
</StructureSection>
 
[[3iep]], [[1lci]] – fLuc – firefly<br />
[[3ier]] – fLuc+PEG 400<br />
[[1ba3]] – fLuc+bromoform<br />
[[3ies]] – fLuc+inhibitor<br />
[[2d1q]] – LcLuc (mutant)+MgATP – ''Luciola cruciata''<br />
[[2d1r]] - LcLuc (mutant)+oxyluciferin+AMP<br />
[[2d1s]], [[2d1t]] - LcLuc (mutant)+intermediate analog<br />
[[3qya]] – Luc (mutant) – ''Lampyris turkestanicus''<br />
[[3fgc]] – VhLuc α+β chain+FMN – ''Vibrio harveyi''<br />
[[1luc]], [[1brl]] - VhLuc α+β chain<br />
[[1bsl]], [[1xkj]] - VhLuc β chain<br />
[[2psd]], [[2psh]], [[2psj]], [[2pse]], [[2psf]] – Luc (mutant) – ''Renilla reniformis''<br />
 
 


==Additional information==
==Additional information==
[[Image:2d1s jena3dviewer image.jpg|left|220px]]
[[Image:2d1s jena3dviewer image.jpg|left|220px]]
<applet load='2d1s' size='450' frame='true' align='right' scene='Luciferase/2d1s/1' caption='Luciferase ([[Luciola cruciata luciferase|2d1s]]) {{Link Toggle FancyCartoonHighQualityView}}.' />
<scene name='Luciferase/2d1s/1'>Luciferase</scene> ([[Luciola cruciata luciferase|2d1s]]) {{Link Toggle FancyCartoonHighQualityView}}
 


Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.
Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.
{{Clear}}
{{Clear}}
*[[Luciola cruciata luciferase]] (Japanese firefly), complexed with Oxyluciferin & AMP ([[2d1r]]) or with an high-energy analogue ([[2d1s]])
 
*[[3ies]],[[3iep]] and [[3ier]] Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124<ref>PMID: 20194791</ref>
*[[1ba3]] firefly luciferase enzyme from ''Photinus pyralis''
*[[2psd]], [[2pse]], [[2psf]], [[2psh]], [[2psj]], and [[2rh7]] the luciferase from ''Renilla reniformis'' (RLuc)
*[[1bsl]] 1.95&Aring; heterodimer of bacterial luciferase from ''Vibrio harveyi''
*[[1brl]] 2.4&Aring; heterodimer of bacterial luciferase from ''Vibrio harveyi''
*[[1xkj]] homodimer of bacterial luciferase from ''Vibrio harveyi''
*[[2d1q]], [[2d1r]] and [[2d1s]] thermostable Japanese Firefly (''Luciola cruciata'') Luciferase complexed with High-energy intermediate analog
*[[2d1t]] thermostable Japanese Firefly Luciferase red-color emission S286N mutant
*[[1lci]] firefly luciferase enzyme from ''Photinus pyralis''
*[[Dinoflagellate luciferase]]
*[[1luc]] The 1.5&Aring; resolution crystal structure of bacterial luciferase from ''Vibrio harveyi''
*[[3fgc]] Bacterial luciferase from ''Vibrio harveyi''<ref>PMID: 19435287</ref>
*[[1vpr]] luciferase of ''Lingulodinium polyedrum'', a marine bioluminescent dinoflagellate
*[[Green Fluorescent Protein]]
* For additional information, see: [[Colored & Bioluminescent Proteins]]
* For additional information, see: [[Colored & Bioluminescent Proteins]]


Latest revision as of 10:42, 11 July 2024

Function

Luciferase (Luc) or luciferin monooxygenase (Lucmo)is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light[1]. See detailed analysis in

See Colored & Bioluminescent Protein.

Relevance

Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success[2]. NanoLuc luciferase is a small mutated luciferase from shrimp with enhanced stability and a significant increase in luminescence [3].

3D Structures of Luciferase

Luciferase 3D structures


Luciferase (PDB code 3iep)

Drag the structure with the mouse to rotate

Additional informationAdditional information

(2d1s)


Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.

ReferencesReferences

  1. ↑ Meighen EA. Molecular biology of bacterial bioluminescence. Microbiol Rev. 1991 Mar;55(1):123-42. PMID:2030669
  2. ↑ Gould SJ, Subramani S. Firefly luciferase as a tool in molecular and cell biology. Anal Biochem. 1988 Nov 15;175(1):5-13. PMID:3072883
  3. ↑ England CG, Ehlerding EB, Cai W. NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. Bioconjug Chem. 2016 May 18;27(5):1175-1187. PMID:27045664 doi:10.1021/acs.bioconjchem.6b00112


Additional LiteratureAdditional Literature

[xtra 1][xtra 2]

  1. ↑ Marques SM, Esteves da Silva JC. Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions. IUBMB Life. 2009 Jan;61(1):6-17. PMID:18949818 doi:10.1002/iub.134
  2. ↑ Inouye S. Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions. Cell Mol Life Sci. 2010 Feb;67(3):387-404. Epub 2009 Oct 27. PMID:19859663 doi:10.1007/s00018-009-0170-8

External ResourcesExternal Resources

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