Luciferase: Difference between revisions

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==See Also==
<StructureSection load='3iep' size='350' side='right' scene='Luciferase/Cv/1' caption='Luciferase (PDB code [[3iep]])'>
*[[Dinoflagellate luciferase]]
== Function ==
*[[Luciola cruciata luciferase]]
[[Luciferase]] (Luc) or '''luciferin monooxygenase''' (Lucmo)is an oxidative enzyme used in bioluminescence.  It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light<ref>PMID:2030669</ref>.  See detailed analysis in<br />
*[[3ies]],[[3iep]] and [[3ier]] Firefly luciferase (FLuc) bound to its natural substrate ATP and the inhibitor PTC124<ref>PMID: 20194791</ref>
* [[Luciola cruciata luciferase]]<br />
*[[1ba3]] firefly luciferase enzyme from ''Photinus pyralis''
* [[Luciferase FMN complex- Vibrio harveyi]]<br />
*[[2psd]], [[2pse]], [[2psf]], [[2psh]], [[2psj]], and [[2rh7]] the luciferase from ''Renilla reniformis'' (RLuc)
* [[Dinoflagellate luciferase]]<br />
*[[1bsl]] 1.95&Aring; heterodimer of bacterial luciferase from ''Vibrio harveyi''
* [[Luciferase (Hebrew)]].
*[[1brl]] 2.4&Aring; heterodimer of bacterial luciferase from ''Vibrio harveyi''
 
*[[1xkj]] homodimer of bacterial luciferase from ''Vibrio harveyi''
See [[Colored & Bioluminescent Protein]].
*[[2d1q]], [[2d1r]] and [[2d1s]] thermostable Japanese Firefly (''Luciola cruciata'') Luciferase complexed with High-energy intermediate analog
 
*[[2d1t]] thermostable Japanese Firefly Luciferase red-color emission S286N mutant
== Relevance ==
*[[1lci]] firefly luciferase enzyme from ''Photinus pyralis''
Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success<ref>PMID:3072883</ref>.  '''NanoLuc luciferase''' is a small mutated luciferase from shrimp with enhanced stability and a significant increase in luminescence <ref>PMID:27045664</ref>.
*[[1luc]] The 1.5&Aring; resolution crystal structure of bacterial luciferase from ''Vibrio harveyi''
 
*[[3fgc]] Bacterial luciferase from ''Vibrio harveyi''<ref>PMID: 19435287</ref>
== 3D Structures of Luciferase ==
*[[1vpr]] luciferase of ''Lingulodinium polyedrum'', a marine bioluminescent dinoflagellate
[[Luciferase 3D structures]]
*[[Green Fluorescent Protein]]
 
*[[Colored & Bioluminescent Proteins]]
</StructureSection>
 
==Additional information==
[[Image:2d1s jena3dviewer image.jpg|left|220px]]
<scene name='Luciferase/2d1s/1'>Luciferase</scene> ([[Luciola cruciata luciferase|2d1s]]) {{Link Toggle FancyCartoonHighQualityView}}
 
 
Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.
{{Clear}}
 
* For additional information, see: [[Colored & Bioluminescent Proteins]]


==References==
==References==
{{Reflist}}
{{Reflist}}


==Additional Literature and Resources==
 
==Additional Literature==
<ref group="xtra">PMID: 18949818</ref><ref group="xtra">PMID: 19859663</ref><references group="xtra"/>
<ref group="xtra">PMID: 18949818</ref><ref group="xtra">PMID: 19859663</ref><references group="xtra"/>
* For additional information, see: [[Colored & Bioluminescent Proteins]]
 
==External Resources==
* [http://news.yahoo.com/s/ap/20100911/ap_on_re_us/us_fireflies_military_research Yahoo news coverage of luciferase studies funded research for military applications]
* [http://news.yahoo.com/s/ap/20100911/ap_on_re_us/us_fireflies_military_research Yahoo news coverage of luciferase studies funded research for military applications]
* [http://en.wikipedia.org/wiki/Luciferase Luciferase at Wikipedia]
* [http://en.wikipedia.org/wiki/Luciferase Luciferase at Wikipedia]
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[[Category: Photoprotein]]
[[Category: Photoprotein]]
[[Category: Alpha/beta-hydrolase]]
[[Category: Alpha/beta-hydrolase]]
[[Category:Topic Page]]

Latest revision as of 10:42, 11 July 2024

Function

Luciferase (Luc) or luciferin monooxygenase (Lucmo)is an oxidative enzyme used in bioluminescence. It catalyzes the oxidation of the pigment luciferin producing oxyluciferin and light[1]. See detailed analysis in

See Colored & Bioluminescent Protein.

Relevance

Luc is used in biological research to assess gene transfection into cells where the Luc gene is added to the transfected gene and the light emission by Luc measured for indication of the transfection success[2]. NanoLuc luciferase is a small mutated luciferase from shrimp with enhanced stability and a significant increase in luminescence [3].

3D Structures of Luciferase

Luciferase 3D structures


Luciferase (PDB code 3iep)

Drag the structure with the mouse to rotate

Additional informationAdditional information

(2d1s)


Several organisms are bioluminescent, meaning that they produce light. Luciferase is one of the enzymes that catalyzes light emitting reactions in organisms.

ReferencesReferences

  1. ↑ Meighen EA. Molecular biology of bacterial bioluminescence. Microbiol Rev. 1991 Mar;55(1):123-42. PMID:2030669
  2. ↑ Gould SJ, Subramani S. Firefly luciferase as a tool in molecular and cell biology. Anal Biochem. 1988 Nov 15;175(1):5-13. PMID:3072883
  3. ↑ England CG, Ehlerding EB, Cai W. NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. Bioconjug Chem. 2016 May 18;27(5):1175-1187. PMID:27045664 doi:10.1021/acs.bioconjchem.6b00112


Additional LiteratureAdditional Literature

[xtra 1][xtra 2]

  1. ↑ Marques SM, Esteves da Silva JC. Firefly bioluminescence: a mechanistic approach of luciferase catalyzed reactions. IUBMB Life. 2009 Jan;61(1):6-17. PMID:18949818 doi:10.1002/iub.134
  2. ↑ Inouye S. Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions. Cell Mol Life Sci. 2010 Feb;67(3):387-404. Epub 2009 Oct 27. PMID:19859663 doi:10.1007/s00018-009-0170-8

External ResourcesExternal Resources

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