1wr6: Difference between revisions

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-[[Image:1wr6.gif|left|200px]]
- {{Structure
+==Crystal structure of GGA3 GAT domain in complex with ubiquitin==
-|PDB= 1wr6 |SIZE=350|CAPTION= <scene name='initialview01'>1wr6</scene>, resolution 2.6&Aring;
+<StructureSection load='1wr6' size='340' side='right'caption='[[1wr6]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND=
+<table><tr><td colspan='2'>[[1wr6]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WR6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WR6 FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wr6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wr6 OCA], [https://pdbe.org/1wr6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wr6 RCSB], [https://www.ebi.ac.uk/pdbsum/1wr6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wr6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GGA3_HUMAN GGA3_HUMAN] Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.<ref>PMID:11301005</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wr/1wr6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wr6 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+GGA (Golgi-localizing, gamma-adaptin ear domain homology, ARF-binding) proteins, which constitute a family of clathrin coat adaptor proteins, have recently been shown to be involved in the ubiquitin-dependent sorting of receptors, through the interaction between the C-terminal three-helix-bundle of the GAT (GGA and Tom1) domain (C-GAT) and ubiquitin. We report here the crystal structure of human GGA3 C-GAT in complex with ubiquitin. A hydrophobic patch on C-GAT helices alpha1 and alpha2 forms a binding site for the hydrophobic Ile44 surface of ubiquitin. Two distinct orientations of ubiquitin Arg42 determine the shape and the charge distribution of ubiquitin Ile44 surface, leading to two different binding modes. Biochemical and NMR data strongly suggest another hydrophobic binding site on C-GAT helices alpha2 and alpha3, opposite to the first binding site, also binds ubiquitin although weakly. The double-sided ubiquitin binding provides the GAT domain with higher efficiency in recognizing ubiquitinated receptors for lysosomal receptor degradation.
-'''Crystal structure of GGA3 GAT domain in complex with ubiquitin'''
+Molecular mechanism of ubiquitin recognition by GGA3 GAT domain.,Kawasaki M, Shiba T, Shiba Y, Yamaguchi Y, Matsugaki N, Igarashi N, Suzuki M, Kato R, Kato K, Nakayama K, Wakatsuki S Genes Cells. 2005 Jul;10(7):639-54. PMID:15966896<ref>PMID:15966896</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1wr6" style="background-color:#fffaf0;"></div>
-==Overview==
+==See Also==
-GGA (Golgi-localizing, gamma-adaptin ear domain homology, ARF-binding) proteins, which constitute a family of clathrin coat adaptor proteins, have recently been shown to be involved in the ubiquitin-dependent sorting of receptors, through the interaction between the C-terminal three-helix-bundle of the GAT (GGA and Tom1) domain (C-GAT) and ubiquitin. We report here the crystal structure of human GGA3 C-GAT in complex with ubiquitin. A hydrophobic patch on C-GAT helices alpha1 and alpha2 forms a binding site for the hydrophobic Ile44 surface of ubiquitin. Two distinct orientations of ubiquitin Arg42 determine the shape and the charge distribution of ubiquitin Ile44 surface, leading to two different binding modes. Biochemical and NMR data strongly suggest another hydrophobic binding site on C-GAT helices alpha2 and alpha3, opposite to the first binding site, also binds ubiquitin although weakly. The double-sided ubiquitin binding provides the GAT domain with higher efficiency in recognizing ubiquitinated receptors for lysosomal receptor degradation.
+*[[3D structures of ubiquitin|3D structures of ubiquitin]]
+== References ==
-==About this Structure==
+<references/>
-WR6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WR6 OCA].
+__TOC__
+</StructureSection>
-==Reference==
-Molecular mechanism of ubiquitin recognition by GGA3 GAT domain., Kawasaki M, Shiba T, Shiba Y, Yamaguchi Y, Matsugaki N, Igarashi N, Suzuki M, Kato R, Kato K, Nakayama K, Wakatsuki S, Genes Cells. 2005 Jul;10(7):639-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15966896 15966896]
 [[Category: Bos taurus]]
 [[Category: Homo sapiens]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Igarashi, N.]]
+[[Category: Igarashi N]]
-[[Category: Kato, K.]]
+[[Category: Kato K]]
-[[Category: Kato, R.]]
+[[Category: Kato R]]
-[[Category: Kawasaki, M.]]
+[[Category: Kawasaki M]]
-[[Category: Matsugaki, N.]]
+[[Category: Matsugaki N]]
-[[Category: Nakayama, K.]]
+[[Category: Nakayama K]]
-[[Category: Shiba, T.]]
+[[Category: Shiba T]]
-[[Category: Shiba, Y.]]
+[[Category: Shiba Y]]
-[[Category: Suzuki, M.]]
+[[Category: Suzuki M]]
-[[Category: Wakatsuki, S.]]
+[[Category: Wakatsuki S]]
-[[Category: Yamaguchi, Y.]]
+[[Category: Yamaguchi Y]]
-[[Category: clathrin coat adaptor protein]]
-[[Category: three-helix bundle]]
-[[Category: ubiquitin-binding protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:00:33 2008''