1ll9: Difference between revisions

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-{{Seed}}
-[[Image:1ll9.png|left|200px]]
-<!--
+==Crystal Structure Of AmpC beta-Lactamase From E. Coli In Complex With Amoxicillin==
-The line below this paragraph, containing "STRUCTURE_1ll9", creates the "Structure Box" on the page.
+<StructureSection load='1ll9' size='340' side='right'caption='[[1ll9]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1ll9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LL9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LL9 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AXL:2-{1-[2-AMINO-2-(4-HYDROXY-PHENYL)-ACETYLAMINO]-2-OXO-ETHYL}-5,5-DIMETHYL-THIAZOLIDINE-4-CARBOXYLIC+ACID'>AXL</scene></td></tr>
-{{STRUCTURE_1ll9|  PDB=1ll9  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ll9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ll9 OCA], [https://pdbe.org/1ll9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ll9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ll9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ll9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AMPC_ECOLI AMPC_ECOLI] This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ll/1ll9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ll9 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+beta-lactamases confer resistance to beta-lactam antibiotics such as penicillins and cephalosporins. However, beta-lactams that form an acyl-intermediate with the enzyme but subsequently are hindered from forming a catalytically competent conformation seem to be inhibitors of beta-lactamases. This inhibition may be imparted by specific groups on the ubiquitous R(1) side chain of beta-lactams, such as the 2-amino-4-thiazolyl methoxyimino (ATMO) group common among third-generation cephalosporins. Using steric hindrance of deacylation as a design guide, penicillin and carbacephem substrates were converted into effective beta-lactamase inhibitors and antiresistance antibiotics. To investigate the structural bases of inhibition, the crystal structures of the acyl-adducts of the penicillin substrate amoxicillin and the new analogous inhibitor ATMO-penicillin were determined. ATMO-penicillin binds in a catalytically incompetent conformation resembling that adopted by third-generation cephalosporins, demonstrating the transferability of such sterically hindered groups in inhibitor design.
-===Crystal Structure Of AmpC beta-Lactamase From E. Coli In Complex With Amoxicillin===
+Using steric hindrance to design new inhibitors of class C beta-lactamases.,Trehan I, Morandi F, Blaszczak LC, Shoichet BK Chem Biol. 2002 Sep;9(9):971-80. PMID:12323371<ref>PMID:12323371</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1ll9" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_12323371}}, adds the Publication Abstract to the page
+*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 12323371 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_12323371}}
+__TOC__
+</StructureSection>
-==About this Structure==
-LL9 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LL9 OCA].
-==Reference==
-<ref group="xtra">PMID:12323371</ref><references group="xtra"/>
-[[Category: Beta-lactamase]]
 [[Category: Escherichia coli]]
-[[Category: Blaszczak, L C.]]
+[[Category: Large Structures]]
-[[Category: Morandi, F.]]
+[[Category: Blaszczak LC]]
-[[Category: Shoichet, B K.]]
+[[Category: Morandi F]]
-[[Category: Trehan, I.]]
+[[Category: Shoichet BK]]
-[[Category: Beta-lactamase]]
+[[Category: Trehan I]]
-[[Category: Cephalosporinase]]
-[[Category: Hydrolase]]
-[[Category: Serine]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:04:26 2009''