6yj6: Difference between revisions
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==Structure of the TFIIIC subcomplex tauA== | |||
<StructureSection load='6yj6' size='340' side='right'caption='[[6yj6]], [[Resolution|resolution]] 3.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6yj6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YJ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YJ6 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yj6 OCA], [https://pdbe.org/6yj6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yj6 RCSB], [https://www.ebi.ac.uk/pdbsum/6yj6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yj6 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/TFC4_YEAST TFC4_YEAST] TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauA domain of TFIIIC that binds boxA DNA promoter sites of tRNA and similar genes. TFC4 is the TFIIIB-assembling subunit of TFIIIC and essential for viability.<ref>PMID:2686985</ref> <ref>PMID:8387209</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Transcription factor (TF) IIIC is a conserved eukaryotic six-subunit protein complex with dual function. It serves as a general TF for most RNA polymerase (Pol) III genes by recruiting TFIIIB, but it is also involved in chromatin organization and regulation of Pol II genes through interaction with CTCF and condensin II. Here, we report the structure of the S. cerevisiae TFIIIC subcomplex tauA, which contains the most conserved subunits of TFIIIC and is responsible for recruitment of TFIIIB and transcription start site (TSS) selection at Pol III genes. We show that tauA binding to its promoter is auto-inhibited by a disordered acidic tail of subunit tau95. We further provide a negative-stain reconstruction of tauA bound to the TFIIIB subunits Brf1 and TBP. This shows that a ruler element in tauA achieves positioning of TFIIIB upstream of the TSS, and suggests remodeling of the complex during assembly of TFIIIB by TFIIIC. | |||
Structure of the TFIIIC subcomplex tauA provides insights into RNA polymerase III pre-initiation complex formation.,Vorlander MK, Jungblut A, Karius K, Baudin F, Grotsch H, Kosinski J, Muller CW Nat Commun. 2020 Sep 30;11(1):4905. doi: 10.1038/s41467-020-18707-y. PMID:32999288<ref>PMID:32999288</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6yj6" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Transcription factor tau|Transcription factor tau]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharomyces cerevisiae]] | |||
[[Category: Muller CW]] | |||
[[Category: Vorlaender MK]] | |||
Latest revision as of 13:21, 22 May 2024
Structure of the TFIIIC subcomplex tauAStructure of the TFIIIC subcomplex tauA
Structural highlights
FunctionTFC4_YEAST TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauA domain of TFIIIC that binds boxA DNA promoter sites of tRNA and similar genes. TFC4 is the TFIIIB-assembling subunit of TFIIIC and essential for viability.[1] [2] Publication Abstract from PubMedTranscription factor (TF) IIIC is a conserved eukaryotic six-subunit protein complex with dual function. It serves as a general TF for most RNA polymerase (Pol) III genes by recruiting TFIIIB, but it is also involved in chromatin organization and regulation of Pol II genes through interaction with CTCF and condensin II. Here, we report the structure of the S. cerevisiae TFIIIC subcomplex tauA, which contains the most conserved subunits of TFIIIC and is responsible for recruitment of TFIIIB and transcription start site (TSS) selection at Pol III genes. We show that tauA binding to its promoter is auto-inhibited by a disordered acidic tail of subunit tau95. We further provide a negative-stain reconstruction of tauA bound to the TFIIIB subunits Brf1 and TBP. This shows that a ruler element in tauA achieves positioning of TFIIIB upstream of the TSS, and suggests remodeling of the complex during assembly of TFIIIB by TFIIIC. Structure of the TFIIIC subcomplex tauA provides insights into RNA polymerase III pre-initiation complex formation.,Vorlander MK, Jungblut A, Karius K, Baudin F, Grotsch H, Kosinski J, Muller CW Nat Commun. 2020 Sep 30;11(1):4905. doi: 10.1038/s41467-020-18707-y. PMID:32999288[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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