1t2s: Difference between revisions
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==Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain== | |||
<StructureSection load='1t2s' size='340' side='right'caption='[[1t2s]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1t2s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T2S FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t2s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2s OCA], [https://pdbe.org/1t2s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t2s RCSB], [https://www.ebi.ac.uk/pdbsum/1t2s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2s ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/AGO2_DROME AGO2_DROME] Essential for RNA interference (RNAi); double-stranded RNA induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger.<ref>PMID:11498593</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t2/1t2s_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t2s ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We describe the solution structures of the Argonaute2 PAZ domain bound to RNA and DNA oligonucleotides. The structures reveal a unique mode of single-stranded nucleic acid binding in which the two 3'-terminal nucleotides are buried in a hydrophobic cleft. We propose that the PAZ domain contributes to the specific recognition of siRNAs by providing a binding pocket for their characteristic two-nucleotide 3' overhangs. | |||
Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain.,Lingel A, Simon B, Izaurralde E, Sattler M Nat Struct Mol Biol. 2004 Jun;11(6):576-7. Epub 2004 May 23. PMID:15156196<ref>PMID:15156196</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1t2s" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
[[ | *[[Argonaute 3D structures|Argonaute 3D structures]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Izaurralde | [[Category: Large Structures]] | ||
[[Category: Lingel | [[Category: Izaurralde E]] | ||
[[Category: Sattler | [[Category: Lingel A]] | ||
[[Category: Simon | [[Category: Sattler M]] | ||
[[Category: Simon B]] | |||
Latest revision as of 12:11, 22 May 2024
Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domainStructural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain
Structural highlights
FunctionAGO2_DROME Essential for RNA interference (RNAi); double-stranded RNA induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedWe describe the solution structures of the Argonaute2 PAZ domain bound to RNA and DNA oligonucleotides. The structures reveal a unique mode of single-stranded nucleic acid binding in which the two 3'-terminal nucleotides are buried in a hydrophobic cleft. We propose that the PAZ domain contributes to the specific recognition of siRNAs by providing a binding pocket for their characteristic two-nucleotide 3' overhangs. Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain.,Lingel A, Simon B, Izaurralde E, Sattler M Nat Struct Mol Biol. 2004 Jun;11(6):576-7. Epub 2004 May 23. PMID:15156196[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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