1zr9: Difference between revisions

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-[[Image:1zr9.png|left|200px]]
-{{STRUCTURE_1zr9|  PDB=1zr9  |  SCENE=  }}
+==Solution Structure of a Human C2H2-type Zinc Finger Protein==
+<StructureSection load='1zr9' size='340' side='right'caption='[[1zr9]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1zr9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZR9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZR9 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zr9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zr9 OCA], [https://pdbe.org/1zr9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zr9 RCSB], [https://www.ebi.ac.uk/pdbsum/1zr9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zr9 ProSAT], [https://www.topsan.org/Proteins/CESG/1zr9 TOPSAN]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ZN593_HUMAN ZN593_HUMAN] Negatively modulates the DNA binding activity of Oct-2 and therefore its transcriptional regulatory activity. Could act either by binding to DNA octamer or by interacting with Oct-2. May also be a modulator of other octamer-binding proteins.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zr/1zr9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zr9 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Here, we report the solution structure of ZNF593, a protein identified in a functional study as a negative modulator of the DNA-binding activity of the Oct-2 transcription factor. ZNF593 contains a classic C(2)H(2) zinc finger domain flanked by about 40 disordered residues on each terminus. Although the protein contains a high degree of intrinsic disorder, the structure of the zinc finger domain was resolved by NMR spectroscopy without a need for N- or C-terminal truncations. The tertiary structure of the zinc finger domain is composed of a beta-hairpin that positions the cysteine side chains for zinc coordination, followed by an atypical kinked alpha-helix containing the two histidine side chain ligands. The structural topology of ZNF593 is similar to a fragment of the double-stranded RNA-binding protein Zfa and the C-terminal zinc finger of MBP-1, a human enhancer binding protein. The structure presented here will provide a guide for future functional studies of how ZNF593 negatively modulates the DNA-binding activity of Oct-2, a POU domain-containing transcription factor. Our work illustrates the unique capacity of NMR spectroscopy for structural analysis of folded domains in a predominantly disordered protein.
-===Solution Structure of a Human C2H2-type Zinc Finger Protein===
+The solution structure of ZNF593 from Homo sapiens reveals a zinc finger in a predominantly unstructured protein.,Hayes PL, Lytle BL, Volkman BF, Peterson FC Protein Sci. 2008 Mar;17(3):571-6. PMID:18287285<ref>PMID:18287285</ref>
-{{ABSTRACT_PUBMED_18287285}}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-==About this Structure==
+<div class="pdbe-citations 1zr9" style="background-color:#fffaf0;"></div>
-[[1zr9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZR9 OCA].
+== References ==
+<references/>
-==Reference==
+__TOC__
-<ref group="xtra">PMID:018287285</ref><references group="xtra"/>
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: CESG, Center for Eukaryotic Structural Genomics.]]
+[[Category: Large Structures]]
-[[Category: Lytle, B L.]]
+[[Category: Lytle BL]]
-[[Category: Peterson, F C.]]
+[[Category: Peterson FC]]
-[[Category: Volkman, B F.]]
+[[Category: Volkman BF]]
-[[Category: Center for eukaryotic structural genomic]]
-[[Category: Cesg]]
-[[Category: Dna binding]]
-[[Category: Protein structure initiative]]
-[[Category: Psi]]
-[[Category: Structural genomic]]
-[[Category: Transcription]]
-[[Category: Zinc finger]]