6fi9: Difference between revisions
m Protected "6fi9" [edit=sysop:move=sysop] |
No edit summary |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==Crystal Structure of a zinc-responsive MarR family member, Lactococcus lactis ZitR== | ||
<StructureSection load='6fi9' size='340' side='right'caption='[[6fi9]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6fi9]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._cremoris_MG1363 Lactococcus lactis subsp. cremoris MG1363]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FI9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FI9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fi9 OCA], [https://pdbe.org/6fi9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fi9 RCSB], [https://www.ebi.ac.uk/pdbsum/6fi9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fi9 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ZITR_LACLM ZITR_LACLM] Zinc-responsive regulator that represses expression of the zit operon in the presence of zinc. Acts by binding two palindromic operator sites overlapping the -35 and -10 boxes of the zit promoter. Could be a sensitive sensor of intracellular zinc to efficiently respond to zinc variations in the environment.<ref>PMID:21317326</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The uptake of zinc, which is vital in trace amounts, is tightly controlled in bacteria. For this control, bacteria of the Streptococcaceae group use a Zn(II)-binding repressor named ZitR in lactococci and AdcR in streptococci, while other bacteria use a Zur protein of the Ferric uptake regulator (Fur) superfamily. ZitR and AdcR proteins, characterized by a winged helix-turn-helix DNA-binding domain, belong to the multiple antibiotic resistance (MarR) superfamily, where they form a specific group of metallo-regulators. Here, one such Zn(II)-responsive repressor, ZitR of Lactococcus lactis subspecies cremoris strain MG1363, is characterized. Size Exclusion Chromatography-coupled to Multi Angle Light Scattering, Circular Dichroism and Isothermal Titration Calorimetry show that purified ZitR is a stable dimer complexed to Zn(II), which is able to bind its two palindromic operator sites on DNA fragments. The crystal structure of ZitR holo-form (Zn(II)4-ZitR2), has been determined at 2.8 A resolution. ZitR is the fourth member of the MarR metallo-regulator subgroup whose structure has been determined. The folding of ZitR/AdcR metallo-proteins is highly conserved between both subspecies (cremoris or lactis) in the Lactococcus lactis species and between species (Lactococcus lactis and Streptococcus pneumoniae or pyogenes) in the Streptococcaceae group. It is also similar to the folding of other MarR members, especially in the DNA-binding domain. Our study contributes to better understand the biochemical and structural properties of metallo-regulators in the MarR superfamily. | |||
Biophysical and structural characterization of a zinc-responsive repressor of the MarR superfamily.,Varela PF, Velours C, Aumont-Nicaise M, Pineau B, Legrand P, Poquet I PLoS One. 2019 Feb 12;14(2):e0210123. doi: 10.1371/journal.pone.0210123., eCollection 2019. PMID:30753183<ref>PMID:30753183</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6fi9" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Lactococcus lactis subsp. cremoris MG1363]] | |||
[[Category: Large Structures]] | |||
[[Category: Legrand P]] | |||
[[Category: Varela PF]] | |||
Latest revision as of 15:28, 9 May 2024
Crystal Structure of a zinc-responsive MarR family member, Lactococcus lactis ZitRCrystal Structure of a zinc-responsive MarR family member, Lactococcus lactis ZitR
Structural highlights
FunctionZITR_LACLM Zinc-responsive regulator that represses expression of the zit operon in the presence of zinc. Acts by binding two palindromic operator sites overlapping the -35 and -10 boxes of the zit promoter. Could be a sensitive sensor of intracellular zinc to efficiently respond to zinc variations in the environment.[1] Publication Abstract from PubMedThe uptake of zinc, which is vital in trace amounts, is tightly controlled in bacteria. For this control, bacteria of the Streptococcaceae group use a Zn(II)-binding repressor named ZitR in lactococci and AdcR in streptococci, while other bacteria use a Zur protein of the Ferric uptake regulator (Fur) superfamily. ZitR and AdcR proteins, characterized by a winged helix-turn-helix DNA-binding domain, belong to the multiple antibiotic resistance (MarR) superfamily, where they form a specific group of metallo-regulators. Here, one such Zn(II)-responsive repressor, ZitR of Lactococcus lactis subspecies cremoris strain MG1363, is characterized. Size Exclusion Chromatography-coupled to Multi Angle Light Scattering, Circular Dichroism and Isothermal Titration Calorimetry show that purified ZitR is a stable dimer complexed to Zn(II), which is able to bind its two palindromic operator sites on DNA fragments. The crystal structure of ZitR holo-form (Zn(II)4-ZitR2), has been determined at 2.8 A resolution. ZitR is the fourth member of the MarR metallo-regulator subgroup whose structure has been determined. The folding of ZitR/AdcR metallo-proteins is highly conserved between both subspecies (cremoris or lactis) in the Lactococcus lactis species and between species (Lactococcus lactis and Streptococcus pneumoniae or pyogenes) in the Streptococcaceae group. It is also similar to the folding of other MarR members, especially in the DNA-binding domain. Our study contributes to better understand the biochemical and structural properties of metallo-regulators in the MarR superfamily. Biophysical and structural characterization of a zinc-responsive repressor of the MarR superfamily.,Varela PF, Velours C, Aumont-Nicaise M, Pineau B, Legrand P, Poquet I PLoS One. 2019 Feb 12;14(2):e0210123. doi: 10.1371/journal.pone.0210123., eCollection 2019. PMID:30753183[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||