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==Human Obscurin Ig57 Domain==
==Human Obscurin Ig57 Domain==
<StructureSection load='6mg9' size='340' side='right'caption='[[6mg9]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='6mg9' size='340' side='right'caption='[[6mg9]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6mg9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MG9 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6mg9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MG9 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">OBSCN, KIAA1556, KIAA1639 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mg9 OCA], [https://pdbe.org/6mg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mg9 RCSB], [https://www.ebi.ac.uk/pdbsum/6mg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mg9 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mg9 OCA], [https://pdbe.org/6mg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mg9 RCSB], [https://www.ebi.ac.uk/pdbsum/6mg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mg9 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/OBSCN_HUMAN OBSCN_HUMAN]] Involved in myofibrillogenesis. Seems to be involved in assembly of myosin into sarcomeric A bands in striated muscle. Isoform 3 together with ANK1 isoform Mu17/Ank1.5 may provide a molecular link between the sarcoplasmic reticulum and myofibrils.<ref>PMID:11448995</ref> <ref>PMID:16205939</ref> 
[https://www.uniprot.org/uniprot/OBSCN_HUMAN OBSCN_HUMAN] Involved in myofibrillogenesis. Seems to be involved in assembly of myosin into sarcomeric A bands in striated muscle. Isoform 3 together with ANK1 isoform Mu17/Ank1.5 may provide a molecular link between the sarcoplasmic reticulum and myofibrils.<ref>PMID:11448995</ref> <ref>PMID:16205939</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Obscurin, a giant modular cytoskeletal protein, is comprised mostly of tandem immunoglobulin-like (Ig-like) domains. This architecture allows obscurin to connect distal targets within the cell. The linkers connecting the Ig domains are usually short (3-4 residues). The physical effect arising from these short linkers is not known; such linkers may lead to a stiff elongated molecule or, conversely, may lead to a more compact and dynamic structure. In an effort to better understand how linkers affect obscurin flexibility, and to better understand the physical underpinnings of this flexibility, here we study the structure and dynamics of four representative sets of dual obscurin Ig domains using experimental and computational techniques. We find in all cases tested that tandem obscurin Ig domains interact at the poles of each domain and tend to stay relatively extended in solution. NMR, SAXS, and MD simulations reveal that while tandem domains are elongated, they also bend and flex significantly. By applying this behavior to a simplified model, it becomes apparent obscurin can link targets more than 200 nm away. However, as targets get further apart, obscurin begins acting as a spring and requires progressively more energy to further elongate.
 
Obscurin is a semi-flexible molecule in solution.,Whitley JA, Ex-Willey AM, Marzolf DR, Ackermann MA, Tongen AL, Kokhan O, Wright NT Protein Sci. 2019 Apr;28(4):717-726. doi: 10.1002/pro.3578. Epub 2019 Feb 6. PMID:30666746<ref>PMID:30666746</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6mg9" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Non-specific serine/threonine protein kinase]]
[[Category: Whitley JA]]
[[Category: Whitley, J A]]
[[Category: Wright NT]]
[[Category: Wright, N T]]
[[Category: Ig domain]]
[[Category: Obscurin]]
[[Category: Structural protein]]
[[Category: Wlc]]

Latest revision as of 10:36, 1 May 2024

Human Obscurin Ig57 DomainHuman Obscurin Ig57 Domain

Structural highlights

6mg9 is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

OBSCN_HUMAN Involved in myofibrillogenesis. Seems to be involved in assembly of myosin into sarcomeric A bands in striated muscle. Isoform 3 together with ANK1 isoform Mu17/Ank1.5 may provide a molecular link between the sarcoplasmic reticulum and myofibrils.[1] [2]

See Also

References

  1. ↑ Young P, Ehler E, Gautel M. Obscurin, a giant sarcomeric Rho guanine nucleotide exchange factor protein involved in sarcomere assembly. J Cell Biol. 2001 Jul 9;154(1):123-36. PMID:11448995
  2. ↑ Borisov AB, Sutter SB, Kontrogianni-Konstantopoulos A, Bloch RJ, Westfall MV, Russell MW. Essential role of obscurin in cardiac myofibrillogenesis and hypertrophic response: evidence from small interfering RNA-mediated gene silencing. Histochem Cell Biol. 2006 Mar;125(3):227-38. Epub 2005 Oct 5. PMID:16205939 doi:http://dx.doi.org/10.1007/s00418-005-0069-x
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