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==Crystal structure of the P.falciparum cytosolic leucyl-tRNA synthetase editing domain (space group P21)==
==Crystal structure of the P.falciparum cytosolic leucyl-tRNA synthetase editing domain (space group P21)==
<StructureSection load='5foc' size='340' side='right' caption='[[5foc]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='5foc' size='340' side='right'caption='[[5foc]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5foc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FOC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FOC FirstGlance]. <br>
<table><tr><td colspan='2'>[[5foc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FOC FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fo4|5fo4]], [[5fod|5fod]], [[5fof|5fof]], [[5fog|5fog]], [[5fol|5fol]], [[5fom|5fom]], [[5fon|5fon]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Leucine--tRNA_ligase Leucine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.4 6.1.1.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5foc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5foc OCA], [https://pdbe.org/5foc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5foc RCSB], [https://www.ebi.ac.uk/pdbsum/5foc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5foc ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5foc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5foc OCA], [http://pdbe.org/5foc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5foc RCSB], [http://www.ebi.ac.uk/pdbsum/5foc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5foc ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/C6KT64_PLAF7 C6KT64_PLAF7]  
There is a need for new antimalarials, ideally with novel mechanisms of action. Benzoxaboroles have been shown to be active against bacteria, fungi, and trypanosomes. Therefore, we investigated the antimalarial activity and mechanism of action of 3-aminomethyl benzoxaboroles against Plasmodium falciparum Two 3-aminomethyl compounds, AN6426 and AN8432, demonstrated good potency against cultured multidrug-resistant (W2 strain) P. falciparum (IC50 310 nM and 490 nM, respectively) and efficacy against murine Plasmodium berghei infection when administered orally once daily for 4 days (ED90 7.4 and 16.2 mg/kg, respectively). To characterize mechanisms of action, we selected parasites with decreased drug sensitivity by culturing with step-wise increases in concentration of AN6426. Resistant clones were characterized by whole genome sequencing. Three generations of resistant parasites had polymorphisms in the predicted editing domain of the gene encoding a P. falciparum leucyl-tRNA synthetase (LeuRS; PF3D7_0622800) and in another gene (PF3D7_1218100), which encodes a protein of unknown function. Solution of the structure of the P. falciparum LeuRS editing domain suggested key roles for mutated residues in LeuRS editing. Short incubations with AN6426 and AN8432, unlike artemisinin, caused dose-dependent inhibition of [14C]leucine incorporation by cultured wild type, but not resistant parasites. The growth of resistant, but not wild type parasites was impaired in the presence of the unnatural amino acid norvaline, consistent with a loss of LeuRS editing activity in resistant parasites. In summary, the benzoxaboroles AN6426 and AN8432 offer effective antimalarial activity, and act, at least in part, against a novel target, the editing domain of P. falciparum LeuRS.


Anti-malarial benzoxaboroles target P. falciparum leucyl-tRNA synthetase.,Sonoiki E, Palencia A, Guo D, Ahyong V, Dong C, Li X, Hernandez VS, Zhang YK, Choi W, Gut J, Legac J, Cooper R, Alley MR, Freund YR, DeRisi J, Cusack S, Rosenthal PJ Antimicrob Agents Chemother. 2016 Jun 6. pii: AAC.00820-16. PMID:27270277<ref>PMID:27270277</ref>
==See Also==
 
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5foc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Leucine--tRNA ligase]]
[[Category: Large Structures]]
[[Category: Ahyong, V]]
[[Category: Plasmodium falciparum 3D7]]
[[Category: Alley, M R.K]]
[[Category: Ahyong V]]
[[Category: Cooper, R]]
[[Category: Alley MRK]]
[[Category: Cusack, S]]
[[Category: Cooper R]]
[[Category: DeRisi, J]]
[[Category: Cusack S]]
[[Category: Dong, C]]
[[Category: DeRisi J]]
[[Category: Freund, Y R]]
[[Category: Dong C]]
[[Category: Guo, D]]
[[Category: Freund YR]]
[[Category: Gut, J]]
[[Category: Guo D]]
[[Category: Hernandez, V S]]
[[Category: Gut J]]
[[Category: Legac, J]]
[[Category: Hernandez VS]]
[[Category: Li, X]]
[[Category: Legac J]]
[[Category: Palencia, A]]
[[Category: Li X]]
[[Category: Rosenthal, P J]]
[[Category: Palencia A]]
[[Category: Sonoiki, E]]
[[Category: Rosenthal PJ]]
[[Category: Aminoacyl-trna synthetase]]
[[Category: Sonoiki E]]
[[Category: Ligase]]
[[Category: Novel boron inhibitors of leur]]
[[Category: P falciparum]]
[[Category: Protein biosynthesis]]

Latest revision as of 10:22, 1 May 2024

Crystal structure of the P.falciparum cytosolic leucyl-tRNA synthetase editing domain (space group P21)Crystal structure of the P.falciparum cytosolic leucyl-tRNA synthetase editing domain (space group P21)

Structural highlights

5foc is a 2 chain structure with sequence from Plasmodium falciparum 3D7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.5Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C6KT64_PLAF7

See Also

5foc, resolution 1.50Å

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