Vinculin: Difference between revisions

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[[Image:Tr.png|left|200px|thumb|Crystal structure of human full-length Vinculin, [[1tr2]]]]
<StructureSection load='1st6' size='340' side='right' caption='Chicken full-length metavinculin, [[1st6]]' scene='' >
{{STRUCTURE_1tr2|  PDB=1tr2  | SIZE=300| SCENE=Vinculin/Cv/2 |right|CAPTION=Human full-length Vinculin, [[1tr2]] }}
== Function ==
[[Vinculin|Vinculins]] (VCLs) are involved in adhesion by linking integrin molecules to the actin cytoskeleton. Its head domain (Vd1) can bind to [[Talin|talin]] or to [[Actinin|alpha-actinin]] at their respective VCL Binding Sites (VBS)<ref>PMID:11152287</ref>.  The protein raver1 RNA Recognition Motif (RRM) forms a complex with VCL or m-VCL.  '''Metavinculin''' (m-VCL) is a splice version of VCL containing an extra ca. 70 amino acids in the C-terminal domain.


[[Vinculin|Vinculins]] (VCLs) are involved in adhesion by linking integrin molecules to the actin cytoskeleton. Its head domain (Vd1) can bind to [[Talin|talin]] or to [[Actinin|alpha-actinin]] at their respective VCL Binding Sites (VBS). A splice variant of vinculin is meta-vinculin (m-VCL). The protein raver1 RNA Recognition Motif (RRM) forms a complex with VCL or m-VCL. The images at the left and at the right correspond to one representative Vinculin structure, ''i.e.'' crystal structure of human full-length Vinculin ([[1tr2]]). <scene name='Sandbox_27/Role_i997_vinculin_head-tail_1/1'>Vinculin Autoinhibition</scene> is achieved through a high affinity intramolecular interaction between tail (orange) and head (aqua) domains ([[1st6]]). Energetically, I997 is key to maintaining this autoinhibition.
== Relevance ==
Loss of VCL could be used as a prognostic factor for colorectal cancer se it promotes metastasis<ref>PMID:25496021</ref>.


== Disease ==
Mutation in m-VCL can yield cardiomyopathic phenotype<ref>PMID:16236538</ref>.
== Structural highlights ==
<scene name='Sandbox_27/Role_i997_vinculin_head-tail_1/1'>Vinculin Autoinhibition</scene> is achieved through a high affinity intramolecular interaction between tail (orange) and head (aqua) domains ([[1st6]]). Energetically, I997 is key to maintaining this autoinhibition.


== 3D Structures of Vinculin ==
== 3D Structures of Vinculin ==
[[Vinculin 3D structures]]


''Update December 2011''
==References==
 
<references />
</StructureSection>
[[Category:Topic Page]]


[[3h2u]] – hVCL Vd1 + raver1 RRM - human<br />
*Created with the participation of [[User:Susan Craig|Susan Craig]].
[[3h2v]] - hVCL C-terminal + raver1 RRM<br />
[[2ibf]], [[2hsq]], [[2gww]] - hVCL Vd1 + SfVCL binding sites from ''Shigella flexneri''<br />
[[1tr2]] – hVCL<br />
[[1ydi]] - hVCL Vd1+hActinin VBS<br />
[[1t01]] - cVCL Vd1+mTalin VBS – chicken<br />
[[1syq]], [[1rkc]], [[1rke]] – hVCL Vd1+hTalin VBS<br />
[[1qkr]] – hVCL C-terminal<br />
 
==Metavinculin== 
 
[[3myi]] – m-VCL tail domain <br />
[[3rf3]] - hm-VCL + invasin IPAA<br />
[[3s90]] - hm-VCL head domain + mTalin-1 residues 1512-1546<br />
[[3tj5]] - hm-VCL head domain + Sca-family protein peptide<br />
[[3tj6]] - hm-VCL head domain + protein Ps 120 peptide<br />
[[2gdc]] – cm-VCL Vd1+SfInvasin C-terminal <br />
[[1xwj]] - cm-VCL Vd1+cTalin VBS3<br />
[[1zvz]], [[1zw2]], [[1zw3]], [[1u6h]] - cm-VCL Vd1+cTalin rod<br />
[[1st6]] – cm-VCL<br />
 
[[Category:Topic Page]]
Created with the participation of [[User:Susan Craig|Susan Craig]].

Latest revision as of 11:07, 19 March 2024

Function

Vinculins (VCLs) are involved in adhesion by linking integrin molecules to the actin cytoskeleton. Its head domain (Vd1) can bind to talin or to alpha-actinin at their respective VCL Binding Sites (VBS)[1]. The protein raver1 RNA Recognition Motif (RRM) forms a complex with VCL or m-VCL. Metavinculin (m-VCL) is a splice version of VCL containing an extra ca. 70 amino acids in the C-terminal domain.

Relevance

Loss of VCL could be used as a prognostic factor for colorectal cancer se it promotes metastasis[2].

Disease

Mutation in m-VCL can yield cardiomyopathic phenotype[3].

Structural highlights

is achieved through a high affinity intramolecular interaction between tail (orange) and head (aqua) domains (1st6). Energetically, I997 is key to maintaining this autoinhibition.

3D Structures of Vinculin

Vinculin 3D structures

References

  1. ↑ Palovuori R, Eskelinen S. Role of vinculin in the maintenance of cell-cell contacts in kidney epithelial MDBK cells. Eur J Cell Biol. 2000 Dec;79(12):961-74. PMID:11152287 doi:http://dx.doi.org/10.1078/0171-9335-00120
  2. ↑ Li T, Guo H, Song Y, Zhao X, Shi Y, Lu Y, Hu S, Nie Y, Fan D, Wu K. Loss of vinculin and membrane-bound beta-catenin promotes metastasis and predicts poor prognosis in colorectal cancer. Mol Cancer. 2014 Dec 11;13:263. doi: 10.1186/1476-4598-13-263. PMID:25496021 doi:http://dx.doi.org/10.1186/1476-4598-13-263
  3. ↑ Vasile VC, Will ML, Ommen SR, Edwards WD, Olson TM, Ackerman MJ. Identification of a metavinculin missense mutation, R975W, associated with both hypertrophic and dilated cardiomyopathy. Mol Genet Metab. 2006 Feb;87(2):169-74. Epub 2005 Oct 19. PMID:16236538 doi:S1096-7192(05)00258-1

Chicken full-length metavinculin, 1st6

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Jaime Prilusky, Michal Harel
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