1cdh: Difference between revisions

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New page: left|200px<br /> <applet load="1cdh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cdh, resolution 2.3Å" /> '''STRUCTURES OF AN HIV...
 
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[[Image:1cdh.gif|left|200px]]<br />
<applet load="1cdh" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1cdh, resolution 2.3&Aring;" />
'''STRUCTURES OF AN HIV AND MHC BINDING FRAGMENT FROM HUMAN CD4 AS REFINED IN TWO CRYSTAL LATTICES'''<br />


==Overview==
==STRUCTURES OF AN HIV AND MHC BINDING FRAGMENT FROM HUMAN CD4 AS REFINED IN TWO CRYSTAL LATTICES==
BACKGROUND: The T-cell surface glycoprotein CD4 interacts with class II, molecules of the major histocompatibility complex (MHC) enhancing the, signal for T-cell activation. Human CD4 also interacts, at high affinity, with the HIV envelope glycoprotein, gp120, to mediate T-cell infection by, HIV. Crystal structures of amino-terminal two-domain (D1D2) fragments of, human CD4, which contain the residues implicated in HIV and MHC, interactions, have been reported earlier. RESULTS: We have determined the, crystal structure of a new D1D2 construct by molecular replacement from a, previously described crystal structure of D1D2. This structure has more, uniform lattice contacts than are in the first. This gives an improved, image of domain D2, which in turn has permitted further refinement of the, initial structure at 2.3 A resolution against a more complete data set., The structure of the second crystal form was also refined at 2.9 A, resolution. In both models, all residues from 1 to 178 are now well, defined, including the loop regions in D2. CONCLUSIONS: Similarities of, the molecular structure in the two lattices suggest that the D1D2 fragment, works as a unit, with segmental flexibility largely restricted to the, junction between domains D2 and D3. Variability of conformation in loops, including those implicated in MHC and HIV binding, requires an 'induced, fit' in these interactions. Well defined density for the exposed side, chain of Phe43 in both crystals confirms a prominent role for this residue, in gp120 binding.
<StructureSection load='1cdh' size='340' side='right'caption='[[1cdh]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1cdh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1cd4 1cd4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CDH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CDH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cdh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cdh OCA], [https://pdbe.org/1cdh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cdh RCSB], [https://www.ebi.ac.uk/pdbsum/1cdh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cdh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CD4_HUMAN CD4_HUMAN] Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cd/1cdh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cdh ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1CDH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure superseeds the now removed PDB entry 1CD4. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CDH OCA].
*[[CD4 3D structures|CD4 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structures of an HIV and MHC binding fragment from human CD4 as refined in two crystal lattices., Ryu SE, Truneh A, Sweet RW, Hendrickson WA, Structure. 1994 Jan 15;2(1):59-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8075984 8075984]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Hendrickson, W.A.]]
[[Category: Hendrickson WA]]
[[Category: Ryu, S.E.]]
[[Category: Ryu SE]]
[[Category: Sweet, R.W.]]
[[Category: Sweet RW]]
[[Category: Truneh, A.]]
[[Category: Truneh A]]
[[Category: t-cell surface glycoprotein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov  8 13:56:21 2007''

Latest revision as of 18:39, 13 March 2024

STRUCTURES OF AN HIV AND MHC BINDING FRAGMENT FROM HUMAN CD4 AS REFINED IN TWO CRYSTAL LATTICESSTRUCTURES OF AN HIV AND MHC BINDING FRAGMENT FROM HUMAN CD4 AS REFINED IN TWO CRYSTAL LATTICES

Structural highlights

1cdh is a 1 chain structure with sequence from Homo sapiens. This structure supersedes the now removed PDB entry 1cd4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CD4_HUMAN Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1cdh, resolution 2.30Å

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