1ar1: Difference between revisions

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New page: left|200px<br /> <applet load="1ar1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ar1, resolution 2.7Å" /> '''STRUCTURE AT 2.7 ANG...
 
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[[Image:1ar1.gif|left|200px]]<br />
<applet load="1ar1" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ar1, resolution 2.7&Aring;" />
'''STRUCTURE AT 2.7 ANGSTROM RESOLUTION OF THE PARACOCCUS DENITRIFICANS TWO-SUBUNIT CYTOCHROME C OXIDASE COMPLEXED WITH AN ANTIBODY FV FRAGMENT'''<br />


==Overview==
==Structure at 2.7 Angstrom Resolution of the Paracoccus Denitrificans two-subunit Cytochrome C Oxidase Complexed with an Antibody Fv Fragment==
The aa3 type cytochrome c oxidase consisting of the core subunits I and II, only was isolated from the soil bacterium Paracoccus denitrificans and, crystallized as complex with a monoclonal antibody Fv fragment. Crystals, could be grown in the presence of a number of different nonionic, detergents. However, only undecyl-beta-D-maltoside and, cyclohexyl-hexyl-beta-D-maltoside yielded well-ordered crystals suitable, for high resolution x-ray crystallographic studies. The crystals belong to, space group P212121 and diffract x-rays to at least 2.5 A (1 A = 0.1 nm), resolution using synchrotron radiation. The structure was determined to a, resolution of 2.7 A using molecular replacement and refined to a, crystallographic R-factor of 20.5% (Rfree = 25.9%). The refined model, includes subunits I ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9380672 (full description)]]
<StructureSection load='1ar1' size='340' side='right'caption='[[1ar1]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ar1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AR1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEA:HEME-A'>HEA</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ar1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ar1 OCA], [https://pdbe.org/1ar1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ar1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ar1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ar1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/COX1B_PARDE COX1B_PARDE] Subunit I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B). This cytochrome c oxidase shows proton pump activity across the membrane in addition to the electron transfer.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ar/1ar1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ar1 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1AR1 is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]] and [[http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]] with CU, MG, CA, HEA and LDA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.9.3.1 1.9.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AR1 OCA]].
*[[Cytochrome c oxidase 3D structures|Cytochrome c oxidase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure at 2.7 A resolution of the Paracoccus denitrificans two-subunit cytochrome c oxidase complexed with an antibody FV fragment., Ostermeier C, Harrenga A, Ermler U, Michel H, Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10547-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9380672 9380672]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Paracoccus denitrificans]]
[[Category: Paracoccus denitrificans]]
[[Category: Protein complex]]
[[Category: Ermler U]]
[[Category: Ermler, U.]]
[[Category: Harrenga A]]
[[Category: Harrenga, A.]]
[[Category: Michel H]]
[[Category: Michel, H.]]
[[Category: Ostermeier C]]
[[Category: Ostermeier, C.]]
[[Category: CA]]
[[Category: CU]]
[[Category: HEA]]
[[Category: LDA]]
[[Category: MG]]
[[Category: antibody complex]]
[[Category: complex (oxidoreductase/antibody)]]
[[Category: cytochrome oxidase]]
[[Category: electron transport]]
[[Category: transmembrane]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 17:55:34 2007''

Latest revision as of 18:27, 13 March 2024

Structure at 2.7 Angstrom Resolution of the Paracoccus Denitrificans two-subunit Cytochrome C Oxidase Complexed with an Antibody Fv FragmentStructure at 2.7 Angstrom Resolution of the Paracoccus Denitrificans two-subunit Cytochrome C Oxidase Complexed with an Antibody Fv Fragment

Structural highlights

1ar1 is a 4 chain structure with sequence from Mus musculus and Paracoccus denitrificans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.7Å
Ligands:, , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

COX1B_PARDE Subunit I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B). This cytochrome c oxidase shows proton pump activity across the membrane in addition to the electron transfer.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1ar1, resolution 2.70Å

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