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==Crystal structure of Aquifex aeolicus methionyl-tRNA synthetase complexed with tRNA(Met)==
==Crystal structure of Aquifex aeolicus methionyl-tRNA synthetase complexed with tRNA(Met)==
<StructureSection load='2csx' size='340' side='right' caption='[[2csx]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
<StructureSection load='2csx' size='340' side='right'caption='[[2csx]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2csx]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CSX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2CSX FirstGlance]. <br>
<table><tr><td colspan='2'>[[2csx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CSX FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ct8|2ct8]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine--tRNA_ligase Methionine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.10 6.1.1.10] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2csx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2csx OCA], [https://pdbe.org/2csx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2csx RCSB], [https://www.ebi.ac.uk/pdbsum/2csx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2csx ProSAT], [https://www.topsan.org/Proteins/RSGI/2csx TOPSAN]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2csx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2csx OCA], [http://pdbe.org/2csx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2csx RCSB], [http://www.ebi.ac.uk/pdbsum/2csx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2csx ProSAT], [http://www.topsan.org/Proteins/RSGI/2csx TOPSAN]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/SYM_AQUAE SYM_AQUAE]] Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (By similarity).  
[https://www.uniprot.org/uniprot/SYM_AQUAE SYM_AQUAE] Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (By similarity).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2csx ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2csx ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In the 2.7-A resolution crystal structure of methionyl-tRNA synthetase (MetRS) in complex with tRNA(Met) and a methionyl-adenylate analog, the tRNA anticodon loop is distorted to form a triple-base stack comprising C34, A35 and A38. A tryptophan residue stacks on C34 to extend the triple-base stack. In addition, C34 forms Watson-Crick-type hydrogen bonds with Arg357. This structure resolves the longstanding question of how MetRS specifically recognizes tRNA(Met).
Structural basis for anticodon recognition by methionyl-tRNA synthetase.,Nakanishi K, Ogiso Y, Nakama T, Fukai S, Nureki O Nat Struct Mol Biol. 2005 Oct;12(10):931-2. Epub 2005 Sep 11. PMID:16155581<ref>PMID:16155581</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2csx" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Aminoacyl tRNA Synthetase|Aminoacyl tRNA Synthetase]]
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
*[[TRNA|TRNA]]
*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aquifex aeolicus huber and stetter 2001]]
[[Category: Aquifex aeolicus]]
[[Category: Methionine--tRNA ligase]]
[[Category: Large Structures]]
[[Category: Fukai, S]]
[[Category: Fukai S]]
[[Category: Nakama, T]]
[[Category: Nakama T]]
[[Category: Nakanishi, K]]
[[Category: Nakanishi K]]
[[Category: Nureki, O]]
[[Category: Nureki O]]
[[Category: Ogiso, Y]]
[[Category: Ogiso Y]]
[[Category: Structural genomic]]
[[Category: Ligase]]
[[Category: Ligase-rna complex]]
[[Category: Rsgi]]

Latest revision as of 16:43, 13 March 2024

Crystal structure of Aquifex aeolicus methionyl-tRNA synthetase complexed with tRNA(Met)Crystal structure of Aquifex aeolicus methionyl-tRNA synthetase complexed with tRNA(Met)

Structural highlights

2csx is a 4 chain structure with sequence from Aquifex aeolicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.7Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

SYM_AQUAE Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2csx, resolution 2.70Å

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