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==Crystal structure of a truncated form of Thermus thermophilus CarH bound to adenosylcobalamin (dark state)==
==Crystal structure of a truncated form of Thermus thermophilus CarH bound to adenosylcobalamin (dark state)==
<StructureSection load='5c8a' size='340' side='right' caption='[[5c8a]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
<StructureSection load='5c8a' size='340' side='right'caption='[[5c8a]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5c8a]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C8A FirstGlance]. <br>
<table><tr><td colspan='2'>[[5c8a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C8A FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5c8e|5c8e]], [[5c8f|5c8f]], [[5c8d|5c8d]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c8a OCA], [http://pdbe.org/5c8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c8a RCSB], [http://www.ebi.ac.uk/pdbsum/5c8a PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c8a OCA], [https://pdbe.org/5c8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c8a RCSB], [https://www.ebi.ac.uk/pdbsum/5c8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c8a ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q746J7_THET2 Q746J7_THET2]
Photoreceptor proteins enable organisms to sense and respond to light. The newly discovered CarH-type photoreceptors use a vitamin B12 derivative, adenosylcobalamin, as the light-sensing chromophore to mediate light-dependent gene regulation. Here we present crystal structures of Thermus thermophilus CarH in all three relevant states: in the dark, both free and bound to operator DNA, and after light exposure. These structures provide visualizations of how adenosylcobalamin mediates CarH tetramer formation in the dark, how this tetramer binds to the promoter -35 element to repress transcription, and how light exposure leads to a large-scale conformational change that activates transcription. In addition to the remarkable functional repurposing of adenosylcobalamin from an enzyme cofactor to a light sensor, we find that nature also repurposed two independent protein modules in assembling CarH. These results expand the biological role of vitamin B12 and provide fundamental insight into a new mode of light-dependent gene regulation.
 
Structural basis for gene regulation by a B-dependent photoreceptor.,Jost M, Fernandez-Zapata J, Polanco MC, Ortiz-Guerrero JM, Chen PY, Kang G, Padmanabhan S, Elias-Arnanz M, Drennan CL Nature. 2015 Sep 28. doi: 10.1038/nature14950. PMID:26416754<ref>PMID:26416754</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5c8a" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Suggestions for new articles|Suggestions for new articles]]
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Drennan, C L]]
[[Category: Large Structures]]
[[Category: Jost, M]]
[[Category: Thermus thermophilus HB27]]
[[Category: Adenosylcobalamin-binding]]
[[Category: Drennan CL]]
[[Category: Dna-binding]]
[[Category: Jost M]]
[[Category: Light sensor]]
[[Category: Transcription]]
[[Category: Transcription factor]]
[[Category: Transcriptional regulator]]

Latest revision as of 15:19, 6 March 2024

Crystal structure of a truncated form of Thermus thermophilus CarH bound to adenosylcobalamin (dark state)Crystal structure of a truncated form of Thermus thermophilus CarH bound to adenosylcobalamin (dark state)

Structural highlights

5c8a is a 4 chain structure with sequence from Thermus thermophilus HB27. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.15Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q746J7_THET2

See Also

5c8a, resolution 2.15Å

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