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==First structure of a proline utilization A proline dehydrogenase domain==
==First structure of a proline utilization A proline dehydrogenase domain==
<StructureSection load='4o8a' size='340' side='right' caption='[[4o8a]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='4o8a' size='340' side='right'caption='[[4o8a]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4o8a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1k87 1k87]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O8A FirstGlance]. <br>
<table><tr><td colspan='2'>[[4o8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1k87 1k87]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O8A FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2OP:(2S)-2-HYDROXYPROPANOIC+ACID'>2OP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b1014, JW0999, poaA, putA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2OP:(2S)-2-HYDROXYPROPANOIC+ACID'>2OP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o8a OCA], [http://pdbe.org/4o8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o8a RCSB], [http://www.ebi.ac.uk/pdbsum/4o8a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o8a ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o8a OCA], [https://pdbe.org/4o8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o8a RCSB], [https://www.ebi.ac.uk/pdbsum/4o8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o8a ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/PUTA_ECOLI PUTA_ECOLI]] Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon.  
[https://www.uniprot.org/uniprot/PUTA_ECOLI PUTA_ECOLI] Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The PutA flavoprotein from Escherichia coli plays multiple roles in proline catabolism by functioning as a membrane-associated bi-functional enzyme and a transcriptional repressor of proline utilization genes. The human homolog of the PutA proline dehydrogenase (PRODH) domain is critical in p53-mediated apoptosis and schizophrenia. Here we report the crystal structure of a 669-residue truncated form of PutA that shows both PRODH and DNA-binding activities, representing the first structure of a PutA protein and a PRODH enzyme from any organism. The structure is a domain-swapped dimer with each subunit comprising three domains: a helical dimerization arm, a 120-residue domain containing a three-helix bundle similar to that in the helix-turn-helix superfamily of DNA-binding proteins and a beta/alpha-barrel PRODH domain with a bound lactate inhibitor. Analysis of the structure provides insight into the mechanism of proline oxidation to pyrroline-5-carboxylate, and functional studies of a mutant protein suggest that the DNA-binding domain is located within the N-terminal 261 residues of E. coli PutA.
 
Structure of the proline dehydrogenase domain of the multifunctional PutA flavoprotein.,Lee YH, Nadaraia S, Gu D, Becker DF, Tanner JJ Nat Struct Biol. 2003 Feb;10(2):109-14. PMID:12514740<ref>PMID:12514740</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4o8a" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Proline utilization A|Proline utilization A]]
*[[Proline utilization A|Proline utilization A]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Ecoli]]
[[Category: Escherichia coli K-12]]
[[Category: Tanner, J J]]
[[Category: Large Structures]]
[[Category: Aldehyde dehydrogenase]]
[[Category: Tanner JJ]]
[[Category: Flavoenzyme]]
[[Category: Oxidoreductase]]
[[Category: Proline dehydrogenase]]
[[Category: Proline utilization some]]
[[Category: Puta]]

Latest revision as of 15:38, 1 March 2024

First structure of a proline utilization A proline dehydrogenase domainFirst structure of a proline utilization A proline dehydrogenase domain

Structural highlights

4o8a is a 1 chain structure with sequence from Escherichia coli K-12. This structure supersedes the now removed PDB entry 1k87. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PUTA_ECOLI Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon.

See Also

4o8a, resolution 2.00Å

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