Proline utilization A

Function Proline utilization A (PutA) or bifunctional protein PutA is a bifunctional flavoprotein which acts as a transcriptional repressor of the put regulon or as a membrane-bound enzyme which catalyzes the oxidation of proline to glutamate. PutA domains include the DNA-binding domain (PRODH), the FAD-dependent proline dehydrogenase domain and the D-pyrroline-5-carboxylate dehydrogenase domain. The binding of proline to the PRODH active site and subsequent reduction of FAD causes a conformation change in PutA and enhance its membrane affinity. As a membrane-bound protein PutA switches from its repressor activity to its enzymatic role. Structural highlights The . Water molecules are shown as red spheres. The 3e2q structure displayed here contains the .

spinqualitylabelsall models E. coli PutA proline dehydrogenase domain with cofactor FAD complex with hydroxyproline (PDB code 3e2q) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

3D Structures of proline utilization A3D Structures of proline utilization A

Updated on 01-February-2022

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• PutA
  • 4nm9 – GsPutA + FAD – Geobacter sulfurreducens
    
  • 7na0 – GsPutA (mutant) + FAD
    
  • 4nma – GsPutA + FAD + tetrahydrofuran derivative
    
  • 2gpe – EcPutA DNA-binding domain
    
  • 2ay0 – EcPutA DNA-binding domain (mutant)
    
  • 4q71, 4q72, 4q73 – BdPutA (mutant) + FAD – Bradyrhizobium diazoefficiens
    
  • 4h6r – DrPutA + FAD – Deinococcus radiodurans
    
  • 2gpe - EcPutA DNA-binding domain - Escherichia coli
    
  • 2jxg, 2jxh - PpPutA DNA-binding domain - Pseudomonas putida - NMR
    
  • 6ufp, 6vz9 – SmPutA (mutant) + FAD – Sinorhizobium melioli
    
• PutA complex
  • 4nmb – GsPutA + FAD + lactate
    
  • 4nmc – GsPutA + FAD + zwittergent
    
  • 4nmd – GsPutA + FAD + dithionite
    
  • 4nme – GsPutA + FAD + propargylglycine
    
  • 4nmf – GsPutA + FAD derivative + menadione bisulfite
    
  • 1tiw – EcPutA PRODH domain + FAD + tetrahydrofuran derivative
    
  • 3e2r, 4jny, 4jnz – EcPutA PRODH domain (mutant) + FAD + tetrahydrofuran derivative
    
  • 1tj0, 1tj1 – EcPutA PRODH domain + FAD + lactate
    
  • 1tj2 – EcPutA PRODH domain + FAD + acetate
    
  • 2fzn – EcPutA PRODH domain + FAD + proline
    
  • 3e2q, 3e2s – EcPutA PRODH domain (mutant) + FAD + proline derivative
    
  • 2fzm – EcPutA PRODH domain + FAD + SO2
    
  • 3itg – EcPutA PRODH domain + FAD + propargylglycine
    
  • 2rbf – EcPutA DNA-binding domain + DNA
    
  • 4o8a – EcPutA DNA-binding + PRODH domains + FAD
    
  • 4h6q – DrPutA + FAD + THF
    
  • 3haz - PutA + FAD + NAD - Bradyrhyzobium japonicum
    
  • 5ux5 - PutA + FAD + NAD - Corynebacterium freiburgense
    
  • 5kf6, 5kf7, 6ufp, 6vz9 - PutA + FAD + NAD + THF - Sinorhizobium meliloti
    
  • 2jxi - PpPutA DNA-binding domain + DNA
    
  • 5ur2 - PutA + FAD derivative + inhibitor - Bdellovibrio bacteriovorus
    
  • 6x9a, 6x9b, 6x9c, 6x9d – SmPutA + FAD + proline derivative
    
  • 6x99 – SmPutA + FAD + Glu derivative
    
  • 7my9, 7mya, 7myb, 7myc – SmPutA + FAD + dithiolane
    
  • 6bsn – BdPutA + FAD + proline