4fpi: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4fpi]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_opacus Rhodococcus opacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FPI FirstGlance]. <br>
<table><tr><td colspan='2'>[[4fpi]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_opacus Rhodococcus opacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FPI FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fpi OCA], [https://pdbe.org/4fpi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fpi RCSB], [https://www.ebi.ac.uk/pdbsum/4fpi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fpi ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fpi OCA], [https://pdbe.org/4fpi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fpi RCSB], [https://www.ebi.ac.uk/pdbsum/4fpi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fpi ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/Q8G9L0_RHOOP Q8G9L0_RHOOP]  
[https://www.uniprot.org/uniprot/Q8G9L0_RHOOP Q8G9L0_RHOOP]  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
5-Chloromuconolactone dehalogenase (5-CMLD) is a unique enzyme that catalyzes the conversion of 5-chloromuconolactone into cis-dienelactone in the new modified ortho-pathway of the 3-chlorocatechol degradation by Rhodococcus opacus 1CP. In all other known chlorocatechol pathways the dehalogenation is a spontaneous secondary reaction of the unstable chloromuconate intermediate following the lactonization process catalyzed by the muconate cycloisomerases. The crystallographic structure of the decameric 5-CMLD was solved by Molecular Replacement, using the coordinates of the low resolution structure of the highly homologous muconolactone isomerase, an enzyme of the conventional ortho-pathway. Muconolactone isomerase catalyzes the endocyclic rearrangement of the double bond within the lactone ring of muconolactone to yield 3-oxoadipate enol lactone. Although both 5-CMLD and muconolactone isomerase share the ability to dechlorinate 5-chloromuconolactone, 5-CMLD shows a significant degree of specialization, having lost the capacity to convert its original substrate muconolactone. The active site of 5-CMLD was previously hypothesized to reside in a deep pocket at the interface of two different subunits, on the basis of a muconolactone isomerase structure analysis. In this study we also performed molecular docking calculations that confirmed these previous findings, and allowed us furthermore to determine the residues involved in the catalytic process.
X-ray crystallographic and molecular docking studies on a unique chloromuconolactone dehalogenase from Rhodococcus opacus 1CP.,Ferraroni M, Kolomytseva M, Golovleva LA, Scozzafava A J Struct Biol. 2013 Apr;182(1):44-50. doi: 10.1016/j.jsb.2013.01.006. Epub 2013, Jan 30. PMID:23376735<ref>PMID:23376735</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4fpi" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Dehalogenase 3D structures|Dehalogenase 3D structures]]
*[[Dehalogenase 3D structures|Dehalogenase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>

Latest revision as of 14:21, 1 March 2024

Crystal Structure of 5-chloromuconolactone isomerase from Rhodococcus opacus 1CPCrystal Structure of 5-chloromuconolactone isomerase from Rhodococcus opacus 1CP

Structural highlights

4fpi is a 20 chain structure with sequence from Rhodococcus opacus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8G9L0_RHOOP

See Also

4fpi, resolution 2.20Å

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