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==Crystal structure of the Salmonella transcriptional regulator SlyA in complex with DNA==
==Crystal structure of the Salmonella transcriptional regulator SlyA in complex with DNA==
<StructureSection load='3q5f' size='340' side='right' caption='[[3q5f]], [[Resolution|resolution]] 2.96&Aring;' scene=''>
<StructureSection load='3q5f' size='340' side='right'caption='[[3q5f]], [[Resolution|resolution]] 2.96&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3q5f]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q5F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q5F FirstGlance]. <br>
<table><tr><td colspan='2'>[[3q5f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q5F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q5F FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qpt|3qpt]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.96&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">slyA, STM1444 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28901 Salmonella enterica])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q5f OCA], [https://pdbe.org/3q5f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q5f RCSB], [https://www.ebi.ac.uk/pdbsum/3q5f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q5f ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q5f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q5f RCSB], [http://www.ebi.ac.uk/pdbsum/3q5f PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/SLYA_SALTY SLYA_SALTY] Transcription regulator that can specifically activate or repress expression of target genes. Required for virulence and survival in the macrophage environment. Probably activates expression of ispA, xseB genes, and of omp operon.<ref>PMID:8544813</ref> <ref>PMID:9284144</ref>  
SlyA is a master virulence regulator that controls the transcription of numerous genes in Salmonella enterica. We present here crystal structures of SlyA by itself and bound to a high-affinity DNA operator sequence in the slyA gene. SlyA interacts with DNA through direct recognition of a guanine base by R65, as well as interactions between conserved R86 and the minor groove and a large network of non-base-specific contacts to the sugar-phosphate backbone. Our structures, together with an unpublished structure of SlyA bound to the drug salicylate (PDB: 3DEU), reveal that unlike many other MarR-family proteins, SlyA dissociates from DNA without large conformational changes when bound to this effector. We propose that SlyA and other MarR global regulators rely more on indirect readout of DNA sequence in order to exert control over many genes, in contrast to proteins such as OhrR which recognize a single operator.
 
Crystal structures of SlyA, a master virulence regulator of Salmonella, in free and DNA-bound states.,Dolan KT, Duguid EM, He C J Biol Chem. 2011 May 5. PMID:21550983<ref>PMID:21550983</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Salmonella enterica]]
[[Category: Large Structures]]
[[Category: Dolan, K T]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
[[Category: Duguid, E M]]
[[Category: Dolan KT]]
[[Category: Marr/slya protein family]]
[[Category: Duguid EM]]
[[Category: Protein-dna complex]]
[[Category: Transcription-dna complex]]
[[Category: Transcriptonal regulator]]
[[Category: Winged helix-turn-helix]]

Latest revision as of 13:45, 21 February 2024

Crystal structure of the Salmonella transcriptional regulator SlyA in complex with DNACrystal structure of the Salmonella transcriptional regulator SlyA in complex with DNA

Structural highlights

3q5f is a 4 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.96Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SLYA_SALTY Transcription regulator that can specifically activate or repress expression of target genes. Required for virulence and survival in the macrophage environment. Probably activates expression of ispA, xseB genes, and of omp operon.[1] [2]

See Also

References

  1. ↑ Ludwig A, Tengel C, Bauer S, Bubert A, Benz R, Mollenkopf HJ, Goebel W. SlyA, a regulatory protein from Salmonella typhimurium, induces a haemolytic and pore-forming protein in Escherichia coli. Mol Gen Genet. 1995 Dec 15;249(5):474-86. PMID:8544813
  2. ↑ Buchmeier N, Bossie S, Chen CY, Fang FC, Guiney DG, Libby SJ. SlyA, a transcriptional regulator of Salmonella typhimurium, is required for resistance to oxidative stress and is expressed in the intracellular environment of macrophages. Infect Immun. 1997 Sep;65(9):3725-30. PMID:9284144

3q5f, resolution 2.96Å

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