3c8q: Difference between revisions

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[[Image:3c8q.png|left|200px]]


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==Contribution of all 20 amino acids at site 96 to the stability and structure of T4 lysozyme==
The line below this paragraph, containing "STRUCTURE_3c8q", creates the "Structure Box" on the page.
<StructureSection load='3c8q' size='340' side='right'caption='[[3c8q]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3c8q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2nzb 2nzb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C8Q FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
{{STRUCTURE_3c8q| PDB=3c8q |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c8q OCA], [https://pdbe.org/3c8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c8q RCSB], [https://www.ebi.ac.uk/pdbsum/3c8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c8q ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/3c8q_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3c8q ConSurf].
<div style="clear:both"></div>


===Contribution of all 20 amino acids at site 96 to the stability and structure of T4 lysozyme===
==See Also==
 
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
 
== References ==
==About this Structure==
<references/>
3C8Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C8Q OCA].
__TOC__
[[Category: Enterobacteria phage t4]]
</StructureSection>
[[Category: Lysozyme]]
[[Category: Escherichia virus T4]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Pdbx_ordinal=, <PDBx:audit_author.]]
[[Category: Mooers BHM]]
[[Category: Antimicrobial]]
[[Category: Bacteriolytic enzyme]]
[[Category: Charge burial]]
[[Category: Electrostatic calculation]]
[[Category: Electrostatic]]
[[Category: Glycosidase]]
[[Category: Hydrogen bonding]]
[[Category: Hydrolase]]
[[Category: Mutational analysis]]
[[Category: Pka shift]]
[[Category: Protein engineering]]
[[Category: Steric strain]]
[[Category: T4 lysozyme]]
[[Category: Thermal stability]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 25 20:10:47 2009''

Latest revision as of 12:33, 21 February 2024

Contribution of all 20 amino acids at site 96 to the stability and structure of T4 lysozymeContribution of all 20 amino acids at site 96 to the stability and structure of T4 lysozyme

Structural highlights

3c8q is a 1 chain structure with sequence from Escherichia virus T4. This structure supersedes the now removed PDB entry 2nzb. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.64Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ENLYS_BPT4 Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Moussa SH, Kuznetsov V, Tran TA, Sacchettini JC, Young R. Protein determinants of phage T4 lysis inhibition. Protein Sci. 2012 Apr;21(4):571-82. doi: 10.1002/pro.2042. Epub 2012 Mar 2. PMID:22389108 doi:http://dx.doi.org/10.1002/pro.2042

3c8q, resolution 1.64Å

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