2qfc: Difference between revisions

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New page: left|200px<br /><applet load="2qfc" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qfc, resolution 2.600Å" /> '''Crystal Structure o...
 
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[[Image:2qfc.jpg|left|200px]]<br /><applet load="2qfc" size="350" color="white" frame="true" align="right" spinBox="true"
caption="2qfc, resolution 2.600&Aring;" />
'''Crystal Structure of Bacillus thuringiensis PlcR complexed with PapR'''<br />


==Overview==
==Crystal Structure of Bacillus thuringiensis PlcR complexed with PapR==
Gram-positive bacteria use a wealth of extracellular signaling peptides, so-called autoinducers, to regulate gene expression according to, population densities. These "quorum sensing" systems control vital, processes such as virulence, sporulation, and gene transfer. Using x-ray, analysis, we determined the structure of PlcR, the major virulence, regulator of the Bacillus cereus group, and obtained mechanistic insights, into the effects of autoinducer binding. Our structural and phylogenetic, analysis further suggests that all of those quorum sensors that bind, directly to their autoinducer peptide derive from a common ancestor and, form a single family (the RNPP family, for Rap/NprR/PlcR/PrgX) with, conserved features. As a consequence, fundamentally different processes in, different bacterial genera appear regulated by essentially the same, autoinducer recognition mechanism. Our results shed light on virulence, control by PlcR and elucidate origin and evolution of multicellular, behavior in bacteria.
<StructureSection load='2qfc' size='340' side='right'caption='[[2qfc]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2qfc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_israelensis_ATCC_35646 Bacillus thuringiensis serovar israelensis ATCC 35646]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QFC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QFC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qfc OCA], [https://pdbe.org/2qfc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qfc RCSB], [https://www.ebi.ac.uk/pdbsum/2qfc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qfc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q3EZ40_BACTI Q3EZ40_BACTI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qf/2qfc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qfc ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2QFC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis] with <scene name='pdbligand=PEG:'>PEG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QFC OCA].
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of PlcR: Insights into virulence regulation and evolution of quorum sensing in Gram-positive bacteria., Declerck N, Bouillaut L, Chaix D, Rugani N, Slamti L, Hoh F, Lereclus D, Arold ST, Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18490-5. Epub 2007 Nov 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17998541 17998541]
[[Category: Bacillus thuringiensis serovar israelensis ATCC 35646]]
[[Category: Bacillus thuringiensis]]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Arold ST]]
[[Category: Arold, S.T.]]
[[Category: Chaix D]]
[[Category: Chaix, D.]]
[[Category: Declerck N]]
[[Category: Declerck, N.]]
[[Category: Hoh F]]
[[Category: Hoh, F.]]
[[Category: Rugani N]]
[[Category: Rugani, N.]]
[[Category: PEG]]
[[Category: tpr; hth]]
[[Category: transcription regulation]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:40:44 2008''

Latest revision as of 12:15, 21 February 2024

Crystal Structure of Bacillus thuringiensis PlcR complexed with PapRCrystal Structure of Bacillus thuringiensis PlcR complexed with PapR

Structural highlights

2qfc is a 4 chain structure with sequence from Bacillus thuringiensis serovar israelensis ATCC 35646. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q3EZ40_BACTI

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2qfc, resolution 2.60Å

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