3nvi: Difference between revisions

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==Structure of N-terminal truncated Nop56/58 bound with L7Ae and box C/D RNA==
==Structure of N-terminal truncated Nop56/58 bound with L7Ae and box C/D RNA==
<StructureSection load='3nvi' size='340' side='right' caption='[[3nvi]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
<StructureSection load='3nvi' size='340' side='right'caption='[[3nvi]], [[Resolution|resolution]] 2.71&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3nvi]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NVI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NVI FirstGlance]. <br>
<table><tr><td colspan='2'>[[3nvi]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NVI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NVI FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nmu|3nmu]], [[3nvk|3nvk]], [[3nvm|3nvm]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.709&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF0060 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 Pyrococcus furiosus DSM 3638]), PF1367, rpl7ae ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=186497 Pyrococcus furiosus DSM 3638])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nvi OCA], [https://pdbe.org/3nvi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nvi RCSB], [https://www.ebi.ac.uk/pdbsum/3nvi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nvi ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nvi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nvi RCSB], [http://www.ebi.ac.uk/pdbsum/3nvi PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q8U4M1_PYRFU Q8U4M1_PYRFU]
Box C/D small nucleolar and Cajal body ribonucleoprotein particles (sno/scaRNPs) direct site-specific 2'-O-methylation of ribosomal and spliceosomal RNAs and are critical for gene expression. Here we report crystal structures of an archaeal box C/D RNP containing three core proteins (fibrillarin, Nop56/58, and L7Ae) and a half-mer box C/D guide RNA paired with a substrate RNA. The structure reveals a guide-substrate RNA duplex orientation imposed by a composite protein surface and the conserved GAEK motif of Nop56/58. Molecular modeling supports a dual C/D RNP structure that closely mimics that recently visualized by electron microscopy. The substrate-bound dual RNP model predicts an asymmetric protein distribution between the RNP that binds and methylates the substrate RNA. The predicted asymmetric nature of the holoenzyme is consistent with previous biochemical data on RNP assembly and provides a simple solution for accommodating base-pairing between the C/D guide RNA and large ribosomal and spliceosomal substrate RNAs.
 
Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle.,Xue S, Wang R, Yang F, Terns RM, Terns MP, Zhang X, Maxwell ES, Li H Mol Cell. 2010 Sep 24;39(6):939-49. PMID:20864039<ref>PMID:20864039</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Ribosomal protein L7|Ribosomal protein L7]]
*[[Kink-turn motif|Kink-turn motif]]
== References ==
*[[Ribosomal protein L7/L12|Ribosomal protein L7/L12]]
<references/>
*[[User:Wayne Decatur/kink-turn motif|User:Wayne Decatur/kink-turn motif]]
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Pyrococcus furiosus dsm 3638]]
[[Category: Large Structures]]
[[Category: Li, H]]
[[Category: Pyrococcus furiosus DSM 3638]]
[[Category: Wang, R]]
[[Category: Li H]]
[[Category: Xue, S]]
[[Category: Wang R]]
[[Category: Kink turn]]
[[Category: Xue S]]
[[Category: Ribosome biogenesis]]
[[Category: Transferase-rna complex]]

Latest revision as of 13:01, 14 February 2024

Structure of N-terminal truncated Nop56/58 bound with L7Ae and box C/D RNAStructure of N-terminal truncated Nop56/58 bound with L7Ae and box C/D RNA

Structural highlights

3nvi is a 6 chain structure with sequence from Pyrococcus furiosus DSM 3638. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.709Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8U4M1_PYRFU

See Also

3nvi, resolution 2.71Å

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