2had: Difference between revisions

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New page: left|200px<br /><applet load="2had" size="450" color="white" frame="true" align="right" spinBox="true" caption="2had, resolution 1.9Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:2had.jpg|left|200px]]<br /><applet load="2had" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2had, resolution 1.9&Aring;" />
'''CRYSTAL STRUCTURE OF HALOALKANE DEHALOGENASE: AN ENZYME TO DETOXIFY HALOGENATED ALKANES'''<br />


==Overview==
==CRYSTAL STRUCTURE OF HALOALKANE DEHALOGENASE: AN ENZYME TO DETOXIFY HALOGENATED ALKANES==
Haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 converts, 1-haloalkanes to the corresponding alcohols and halide ions with water as, the sole cosubstrate and without any need for oxygen or cofactors. The, three-dimensional structure has been determined by multiple isomorphous, replacement techniques using three heavy atom derivatives. The structure, has been refined at 2.4 A resolution to an R-factor of 17.9%. The, monomeric enzyme is a spherical molecule and is composed to two domains:, domain I has an alpha/beta type structure with a central eight-stranded, mainly parallel beta-sheet. Domain II lies like a cap on top of domain I, and consists of alpha-helices connected by loops. Except for the cap, domain the structure resembles that of the dienelactone hydrolase in spite, of any significant sequence homology. The putative active site is, completely buried in an internal hydrophobic cavity which is located, between the two domains. From the analysis of the structure it is, suggested that Asp124 is the nucleophilic residue essential for the, catalysis. It interacts with His289 which is hydrogen-bonded to Asp260.
<StructureSection load='2had' size='340' side='right'caption='[[2had]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2had]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthobacter_autotrophicus Xanthobacter autotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HAD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HAD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2had FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2had OCA], [https://pdbe.org/2had PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2had RCSB], [https://www.ebi.ac.uk/pdbsum/2had PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2had ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DHLA_XANAU DHLA_XANAU] Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes terminally mono- and di- chlorinated and brominated alkanes (up to C4 only). The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ha/2had_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2had ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2HAD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xanthobacter_autotrophicus Xanthobacter autotrophicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HAD OCA].
*[[Dehalogenase 3D structures|Dehalogenase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of haloalkane dehalogenase: an enzyme to detoxify halogenated alkanes., Franken SM, Rozeboom HJ, Kalk KH, Dijkstra BW, EMBO J. 1991 Jun;10(6):1297-302. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2026135 2026135]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Xanthobacter autotrophicus]]
[[Category: Xanthobacter autotrophicus]]
[[Category: Dijkstra, B.W.]]
[[Category: Dijkstra BW]]
[[Category: Franken, S.M.]]
[[Category: Franken SM]]
[[Category: Verschueren, K.H.G.]]
[[Category: Verschueren KHG]]
[[Category: dehalogenase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:38:32 2007''

Latest revision as of 12:31, 14 February 2024

CRYSTAL STRUCTURE OF HALOALKANE DEHALOGENASE: AN ENZYME TO DETOXIFY HALOGENATED ALKANESCRYSTAL STRUCTURE OF HALOALKANE DEHALOGENASE: AN ENZYME TO DETOXIFY HALOGENATED ALKANES

Structural highlights

2had is a 1 chain structure with sequence from Xanthobacter autotrophicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DHLA_XANAU Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes terminally mono- and di- chlorinated and brominated alkanes (up to C4 only). The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2had, resolution 1.90Å

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