1zv1: Difference between revisions

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New page: left|200px<br /><applet load="1zv1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zv1, resolution 1.60Å" /> '''Crystal structure of...
 
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[[Image:1zv1.gif|left|200px]]<br /><applet load="1zv1" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1zv1, resolution 1.60&Aring;" />
'''Crystal structure of the dimerization domain of doublesex protein from D. melanogaster'''<br />


==Overview==
==Crystal structure of the dimerization domain of doublesex protein from D. melanogaster==
Male- and female-specific isoforms of the Doublesex (DSX) transcription, factor regulate somatic sexual differentiation in Drosophila. The isoforms, (DSX(M) and DSX(F)) share an N-terminal DNA binding domain (the DM motif), broadly conserved among metazoan sex-determining pathways. DM-DNA, recognition is enhanced by a C-terminal dimerization domain. The crystal, structure of this domain, determined at a resolution of 1.6 A, reveals a, novel dimeric arrangement of ubiquitin-associated (UBA) folds. Although, this alpha-helical motif is well characterized in pathways of DNA repair, and subcellular trafficking, to our knowledge this is its first report in, a transcription factor. Dimerization is mediated by a non-canonical, hydrophobic interface extrinsic to the putative ubiquitin binding surface., Key side chains at this interface, identified by alanine scanning, mutagenesis, are conserved among DSX homologs. The mechanism of, dimerization is thus unrelated to the low affinity domain swapping, observed among ubiquitin-associated CUE domains. The unexpected, observation of a ubiquitin-associated fold in DSX extends the repertoire, of alpha-helical dimerization elements in transcription factors. The, possibility that the ubiquitination machinery participates in the, regulation of sexual dimorphism is discussed.
<StructureSection load='1zv1' size='340' side='right'caption='[[1zv1]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1zv1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZV1 FirstGlance]. <br>
1ZV1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZV1 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zv1 OCA], [https://pdbe.org/1zv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zv1 RCSB], [https://www.ebi.ac.uk/pdbsum/1zv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zv1 ProSAT]</span></td></tr>
==Reference==
</table>
Dimerization of doublesex is mediated by a cryptic ubiquitin-associated domain fold: implications for sex-specific gene regulation., Bayrer JR, Zhang W, Weiss MA, J Biol Chem. 2005 Sep 23;280(38):32989-96. Epub 2005 Jul 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16049008 16049008]
== Function ==
[https://www.uniprot.org/uniprot/DSX_DROME DSX_DROME] Controls somatic sexual differentiation. Binds directly and specifically to the FBE (fat body enhancer) of the yolk protein 1 and 2 genes (Yp1 and Yp2). This enhancer is sufficient to direct the female-specific transcription characteristic of the Yp genes in adult fat bodies. Involved in regulation of male-specific expression of takeout in brain-associated fat body.<ref>PMID:12435630</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zv/1zv1_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zv1 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bayrer, J.R.]]
[[Category: Bayrer JR]]
[[Category: Li, B.]]
[[Category: Li B]]
[[Category: Phillips, N.B.]]
[[Category: Phillips NB]]
[[Category: Wan, Z.]]
[[Category: Wan Z]]
[[Category: Weiss, M.A.]]
[[Category: Weiss MA]]
[[Category: dimerization]]
[[Category: doublesex]]
[[Category: sex determination]]
[[Category: transcription factor]]
[[Category: uba domain]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:40:45 2007''

Latest revision as of 12:06, 14 February 2024

Crystal structure of the dimerization domain of doublesex protein from D. melanogasterCrystal structure of the dimerization domain of doublesex protein from D. melanogaster

Structural highlights

1zv1 is a 2 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DSX_DROME Controls somatic sexual differentiation. Binds directly and specifically to the FBE (fat body enhancer) of the yolk protein 1 and 2 genes (Yp1 and Yp2). This enhancer is sufficient to direct the female-specific transcription characteristic of the Yp genes in adult fat bodies. Involved in regulation of male-specific expression of takeout in brain-associated fat body.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Dauwalder B, Tsujimoto S, Moss J, Mattox W. The Drosophila takeout gene is regulated by the somatic sex-determination pathway and affects male courtship behavior. Genes Dev. 2002 Nov 15;16(22):2879-92. PMID:12435630 doi:http://dx.doi.org/10.1101/gad.1010302

1zv1, resolution 1.60Å

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