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[[Image:1rpu.png|left|200px]]


{{STRUCTURE_1rpu|  PDB=1rpu  |  SCENE=  }}
==Crystal Structure of CIRV p19 bound to siRNA==
 
<StructureSection load='1rpu' size='340' side='right'caption='[[1rpu]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
===Crystal Structure of CIRV p19 bound to siRNA===
== Structural highlights ==
 
<table><tr><td colspan='2'>[[1rpu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Carnation_Italian_ringspot_virus Carnation Italian ringspot virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RPU FirstGlance]. <br>
{{ABSTRACT_PUBMED_14697199}}
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rpu OCA], [https://pdbe.org/1rpu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rpu RCSB], [https://www.ebi.ac.uk/pdbsum/1rpu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rpu ProSAT]</span></td></tr>
==About this Structure==
</table>
[[1rpu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Carnation_italian_ringspot_virus Carnation italian ringspot virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RPU OCA].  
== Function ==
[https://www.uniprot.org/uniprot/P19_CIRV P19_CIRV] Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. Binds to short interfering RNAs (siRNAs) with high affinity. Acts as a molecular caliper to specifically select siRNAs based on the length of the duplex region of the RNA.<ref>PMID:14697199</ref> <ref>PMID:19254536</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rp/1rpu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rpu ConSurf].
<div style="clear:both"></div>


==See Also==
==See Also==
*[[Alignment of 1rpu vs 1r9f|Alignment of 1rpu vs 1r9f]]
*[[Flock house virus protein B2|Flock house virus protein B2]]
*[[Flock house virus B2 protein Suppression of RNA Silencing|Flock house virus B2 protein Suppression of RNA Silencing]]
*[[P19|P19]]
*[[P19|P19]]
*[[RNA Interference|RNA Interference]]
*[[RNA silencing suppressor|RNA silencing suppressor]]
*[[Suppression of RNA Silencing by Viruses|Suppression of RNA Silencing by Viruses]]
*[[Suppression of RNA Silencing by Viruses|Suppression of RNA Silencing by Viruses]]
*[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]]
*[[Tomato aspermy virus protein 2b Suppression of RNA Silencing|Tomato aspermy virus protein 2b Suppression of RNA Silencing]]
*[[User:Wayne Decatur/Chem791A Work Session|User:Wayne Decatur/Chem791A Work Session]]
== References ==
*[[User:Wayne Decatur/Flock house virus B2 protein Suppression of RNA Silencing|User:Wayne Decatur/Flock house virus B2 protein Suppression of RNA Silencing]]
<references/>
*[[User:Wayne Decatur/Plant Viral Protein p19 Suppression of RNA Silencing|User:Wayne Decatur/Plant Viral Protein p19 Suppression of RNA Silencing]]
__TOC__
*[[User:Wayne Decatur/Suppression of RNA Silencing by Viruses|User:Wayne Decatur/Suppression of RNA Silencing by Viruses]]
</StructureSection>
*[[User:Wayne Decatur/Teaching Proteopedia|User:Wayne Decatur/Teaching Proteopedia]]
[[Category: Carnation Italian ringspot virus]]
*[[User:Wayne Decatur/Tomato aspermy virus protein 2b Suppression of RNA Silencing|User:Wayne Decatur/Tomato aspermy virus protein 2b Suppression of RNA Silencing]]
[[Category: Large Structures]]
 
[[Category: Burgyan J]]
==Reference==
[[Category: Hall TMT]]
<ref group="xtra">PMID:014697199</ref><references group="xtra"/>
[[Category: Szittya G]]
[[Category: Carnation italian ringspot virus]]
[[Category: Vargason JM]]
[[Category: Burgyan, J.]]
[[Category: Hall, T M.T.]]
[[Category: Szittya, G.]]
[[Category: Vargason, J M.]]
[[Category: Protein-rna complex]]
[[Category: Rna binding protein-rna complex]]
[[Category: Rna double helix]]
[[Category: Rna length recognition]]
[[Category: Rnai]]

Latest revision as of 11:24, 14 February 2024

Crystal Structure of CIRV p19 bound to siRNACrystal Structure of CIRV p19 bound to siRNA

Structural highlights

1rpu is a 4 chain structure with sequence from Carnation Italian ringspot virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

P19_CIRV Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. Binds to short interfering RNAs (siRNAs) with high affinity. Acts as a molecular caliper to specifically select siRNAs based on the length of the duplex region of the RNA.[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Vargason JM, Szittya G, Burgyan J, Tanaka Hall TM. Size selective recognition of siRNA by an RNA silencing suppressor. Cell. 2003 Dec 26;115(7):799-811. PMID:14697199
  2. ↑ Xia Z, Zhu Z, Zhu J, Zhou R. Recognition mechanism of siRNA by viral p19 suppressor of RNA silencing: a molecular dynamics study. Biophys J. 2009 Mar 4;96(5):1761-9. doi: 10.1016/j.bpj.2008.11.047. PMID:19254536 doi:http://dx.doi.org/10.1016/j.bpj.2008.11.047

1rpu, resolution 2.50Å

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