1rpu

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Crystal Structure of CIRV p19 bound to siRNACrystal Structure of CIRV p19 bound to siRNA

Structural highlights

1rpu is a 4 chain structure with sequence from Carnation Italian ringspot virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

P19_CIRV Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. Binds to short interfering RNAs (siRNAs) with high affinity. Acts as a molecular caliper to specifically select siRNAs based on the length of the duplex region of the RNA.[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. Vargason JM, Szittya G, Burgyan J, Tanaka Hall TM. Size selective recognition of siRNA by an RNA silencing suppressor. Cell. 2003 Dec 26;115(7):799-811. PMID:14697199
  2. Xia Z, Zhu Z, Zhu J, Zhou R. Recognition mechanism of siRNA by viral p19 suppressor of RNA silencing: a molecular dynamics study. Biophys J. 2009 Mar 4;96(5):1761-9. doi: 10.1016/j.bpj.2008.11.047. PMID:19254536 doi:http://dx.doi.org/10.1016/j.bpj.2008.11.047

1rpu, resolution 2.50Å

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OCA