1rku: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1rku.jpg|left|200px]]<br /><applet load="1rku" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1rku, resolution 1.47&Aring;" />
-'''Crystal Structure of ThrH gene product of Pseudomonas Aeruginosa'''<br />
-==Overview==
+==Crystal Structure of ThrH gene product of Pseudomonas Aeruginosa==
-The thrH gene product of Pseudomonas aeruginosa has been shown to, complement both homoserine kinase (thrB gene product) and phosphoserine, phosphatase (serB gene product) activities in vivo. Sequence comparison, has revealed that ThrH is related to phosphoserine phosphatases (PSP, EC, 3.1.3.3) and belongs to the l-2-haloacid dehalogenase-like protein, superfamily. We have solved the crystal structures of ThrH in the apoform, and in complex with a bound product phosphate. The structure confirms an, overall fold similar to that of PSP. Most of the catalytic residues of PSP, are also conserved in ThrH, suggesting that similar catalytic mechanisms, are used by both enzymes. Spectrophotometry-based in vitro assays show, that ThrH is indeed a phosphoserine phosphatase with a K(m) of 0.207 mm, and k(cat) of 13.4 min(-1), comparable with those of other PSPs. More, interestingly, using high pressure liquid chromatography-based assays, we, have demonstrated that ThrH is able to further transfer the phosphoryl, group to homoserine using phosphoserine as the phosphoryl group donor, indicating that ThrH has a novel phosphoserine:homoserine, phosphotransferase activity.
+<StructureSection load='1rku' size='340' side='right'caption='[[1rku]], [[Resolution|resolution]] 1.47&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1rku]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RKU FirstGlance]. <br>
-RKU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1] with MG and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RKU OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.47&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rku OCA], [https://pdbe.org/1rku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rku RCSB], [https://www.ebi.ac.uk/pdbsum/1rku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rku ProSAT]</span></td></tr>
-The thrH gene product of Pseudomonas aeruginosa is a dual activity enzyme with a novel phosphoserine:homoserine phosphotransferase activity., Singh SK, Yang K, Karthikeyan S, Huynh T, Zhang X, Phillips MA, Zhang H, J Biol Chem. 2004 Mar 26;279(13):13166-73. Epub 2003 Dec 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14699121 14699121]
+</table>
-[[Category: Pseudomonas aeruginosa pao1]]
+== Function ==
-[[Category: Single protein]]
+[https://www.uniprot.org/uniprot/THRH_PSEAE THRH_PSEAE] Phosphoserine phosphatase that mediates dephosphorylation of phosphoserine in the serine biosynthesis pathway. Also able to dephosphorylate other substrates such as phospho-L(or D)-threonine, with lower activity. Shows phosphoserine:homoserine phosphotransferase activity by transferring the phosphoryl group to homoserine using phosphoserine as the phosphoryl group donor.<ref>PMID:10220164</ref> <ref>PMID:14699121</ref>
-[[Category: Huynh, T.]]
+== Evolutionary Conservation ==
-[[Category: Karthikeyan, S.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Phillips, M.A.]]
+Check<jmol>
-[[Category: Singh, S.K.]]
+  <jmolCheckbox>
-[[Category: Subramanian, K.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rk/1rku_consurf.spt"</scriptWhenChecked>
-[[Category: Yang, K.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: Zhang, H.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: Zhang, X.]]
+  </jmolCheckbox>
-[[Category: EDO]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rku ConSurf].
-[[Category: MG]]
+<div style="clear:both"></div>
-[[Category: phosphoserine phosphatase]]
+== References ==
-[[Category: phosphoserine phosphoryl donor]]
+<references/>
-[[Category: phosphoserine:homoserine phosphotransferase]]
+__TOC__
-[[Category: structure]]
+</StructureSection>
-[[Category: thrh]]
+[[Category: Large Structures]]
+[[Category: Pseudomonas aeruginosa PAO1]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:51:25 2007''
+[[Category: Huynh T]]
+[[Category: Karthikeyan S]]
+[[Category: Phillips MA]]
+[[Category: Singh SK]]
+[[Category: Subramanian K]]
+[[Category: Yang K]]
+[[Category: Zhang H]]
+[[Category: Zhang X]]