1qqt: Difference between revisions

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[[Image:1qqt.gif|left|200px]]


{{Structure
==METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI==
|PDB= 1qqt |SIZE=350|CAPTION= <scene name='initialview01'>1qqt</scene>, resolution 2.03&Aring;
<StructureSection load='1qqt' size='340' side='right'caption='[[1qqt]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
<table><tr><td colspan='2'>[[1qqt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QQT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QQT FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Methionine--tRNA_ligase Methionine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.10 6.1.1.10]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qqt OCA], [https://pdbe.org/1qqt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qqt RCSB], [https://www.ebi.ac.uk/pdbsum/1qqt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qqt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SYM_ECOLI SYM_ECOLI] Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.[HAMAP-Rule:MF_00098]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qq/1qqt_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qqt ConSurf].
<div style="clear:both"></div>


'''METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI'''
==See Also==
 
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
The 3D structure of monomeric C-truncated Escherichia coli methionyl-tRNA synthetase, a class 1 aminoacyl-tRNA synthetase, has been solved at 2.0 A resolution. Remarkably, the polypeptide connecting the two halves of the Rossmann fold exposes two identical knuckles related by a 2-fold axis but with zinc in the distal knuckle only. Examination of available MetRS orthologs reveals four classes according to the number and zinc content of the putative knuckles. Extreme cases are exemplified by the MetRS of eucaryotic or archaeal origin, where two knuckles and two metal ions are expected, and by the mitochondrial enzymes, which are predicted to have one knuckle without metal ion.
 
==About this Structure==
1QQT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QQT OCA].
 
==Reference==
Crystal structure of Escherichia coli methionyl-tRNA synthetase highlights species-specific features., Mechulam Y, Schmitt E, Maveyraud L, Zelwer C, Nureki O, Yokoyama S, Konno M, Blanquet S, J Mol Biol. 1999 Dec 17;294(5):1287-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10600385 10600385]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Methionine--tRNA ligase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Blanquet S]]
[[Category: Blanquet, S.]]
[[Category: Konno M]]
[[Category: Konno, M.]]
[[Category: Maveyraud L]]
[[Category: Maveyraud, L.]]
[[Category: Mechulam Y]]
[[Category: Mechulam, Y.]]
[[Category: Nureki O]]
[[Category: Nureki, O.]]
[[Category: Schmitt E]]
[[Category: Schmitt, E.]]
[[Category: Yokoyama S]]
[[Category: Yokoyama, S.]]
[[Category: Zelwer C]]
[[Category: Zelwer, C.]]
[[Category: ZN]]
[[Category: helix bundle]]
[[Category: rossmann fold]]
[[Category: trna ligase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:41:28 2008''

Latest revision as of 11:16, 14 February 2024

METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLIMETHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI

Structural highlights

1qqt is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.03Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SYM_ECOLI Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.[HAMAP-Rule:MF_00098]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1qqt, resolution 2.03Å

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