1pys: Difference between revisions

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New page: left|200px<br /><applet load="1pys" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pys, resolution 2.9Å" /> '''PHENYLALANYL-TRNA SYN...
 
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[[Image:1pys.jpg|left|200px]]<br /><applet load="1pys" size="450" color="white" frame="true" align="right" spinBox="true"
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'''PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS'''<br />


==Overview==
==PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS==
The crystal structure of phenylalanyl-tRNA synthetase from Thermus, thermophilus, solved at 2.9 A resolution, displays (alpha beta)2 subunit, organization. Unexpectedly, both the catalytic alpha- and the, non-catalytic beta-subunits comprise the characteristic fold of the class, II active-site domains. The alpha beta heterodimer contains most of the, building blocks so far identified in the class II synthetases. The, presence of an RNA-binding domain, similar to that of the U1A spliceosomal, protein, in the beta-subunit is indicative of structural relationships, among different families of RNA-binding proteins. The structure suggests a, plausible catalytic mechanism which explains why the primary site of tRNA, aminoacylation is different from that of the other class II enzymes.
<StructureSection load='1pys' size='340' side='right'caption='[[1pys]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1pys]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PYS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pys OCA], [https://pdbe.org/1pys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pys RCSB], [https://www.ebi.ac.uk/pdbsum/1pys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pys ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SYFA_THET8 SYFA_THET8]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/py/1pys_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pys ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1PYS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phenylalanine--tRNA_ligase Phenylalanine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.20 6.1.1.20] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PYS OCA].
*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structure of phenylalanyl-tRNA synthetase from Thermus thermophilus., Mosyak L, Reshetnikova L, Goldgur Y, Delarue M, Safro MG, Nat Struct Biol. 1995 Jul;2(7):537-47. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7664121 7664121]
[[Category: Large Structures]]
[[Category: Phenylalanine--tRNA ligase]]
[[Category: Thermus thermophilus HB8]]
[[Category: Protein complex]]
[[Category: Delarue M]]
[[Category: Thermus thermophilus]]
[[Category: Goldgur Y]]
[[Category: Delarue, M.]]
[[Category: Mosyak L]]
[[Category: Goldgur, Y.]]
[[Category: Reshetnikova L]]
[[Category: Mosyak, L.]]
[[Category: Safro M]]
[[Category: Reshetnikova, L.]]
[[Category: Safro, M.]]
[[Category: MG]]
[[Category: class ii aminoacyl-trna synthetase]]
[[Category: helix-turn-helix motif]]
[[Category: phenylalanyl-trna synthetase]]
[[Category: rbd domain]]
[[Category: sh3 domain]]
[[Category: thermus thermophilus]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:14:25 2007''

Latest revision as of 11:11, 14 February 2024

PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUSPHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS

Structural highlights

1pys is a 2 chain structure with sequence from Thermus thermophilus HB8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.9Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SYFA_THET8

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1pys, resolution 2.90Å

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