1p4x: Difference between revisions

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-[[Image:1p4x.jpg|left|200px]]
- {{Structure
+==Crystal structure of SarS protein from Staphylococcus Aureus==
-|PDB= 1p4x |SIZE=350|CAPTION= <scene name='initialview01'>1p4x</scene>, resolution 2.20&Aring;
+<StructureSection load='1p4x' size='340' side='right'caption='[[1p4x]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND=
+<table><tr><td colspan='2'>[[1p4x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P4X FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-|GENE=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p4x OCA], [https://pdbe.org/1p4x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p4x RCSB], [https://www.ebi.ac.uk/pdbsum/1p4x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p4x ProSAT]</span></td></tr>
-|DOMAIN=
+</table>
-|RELATEDENTRY=[[1hsj|1HSJ]]
+== Function ==
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p4x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p4x OCA], [http://www.ebi.ac.uk/pdbsum/1p4x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p4x RCSB]</span>
+[https://www.uniprot.org/uniprot/SARS_STAA8 SARS_STAA8] Transcriptional regulator that controls expression of some virulence factors in a cell density-dependent manner. Acts as an activator of the gene encoding protein A (spa). Negatively regulates the expression of alpha-hemolysin (hla).<ref>PMID:10931334</ref> <ref>PMID:11254606</ref>
-}}
+== References ==
+<references/>
-'''Crystal structure of SarS protein from Staphylococcus Aureus'''
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
-==Overview==
-The expression of virulence determinants in Staphylococcus aureus is controlled by global regulatory loci (e.g., sarA and agr). One of these determinants, protein A (spa), is activated by sarS, which encodes a 250-residue DNA-binding protein. Genetic analysis indicated that the agr locus likely mediates spa repression by suppressing the transcription of sarS. Contrary to SarA and SarR, which require homodimer formation for proper function, SarS is unusual within the SarA protein family in that it contains two homologous halves, with each half sharing sequence similarity to SarA and SarR. Here we report the 2.2 A resolution X-ray crystal structure of the SarS protein. SarS has folds similar to those of SarR and, quite plausibly, the native SarA structure. Two typical winged-helix DNA-binding domains are connected by a well-ordered loop. The interactions between the two domains are extensive and conserved. The putative DNA-binding surface is highly positively charged. In contrast, negatively charged patches are located opposite to the DNA-binding surface. Furthermore, sequence alignment and structural comparison revealed that MarR has folds similar to those of SarR and SarS. Members of the MarR protein family have previously been implicated in the negative regulation of an efflux pump involved in multiple antibiotic resistance in many gram-negative species. We propose that MarR also belongs to the winged-helix protein family and has a similar mode of DNA binding as SarR and SarS and possibly the entire SarA protein family member. Based on the structural differences of SarR, SarS, and MarR, we further classified these winged-helix proteins to three subfamilies, SarA, SarS, and MarR. Finally, a possible transcription regulation mechanism is proposed.
-==About this Structure==
-P4X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4X OCA].
-==Reference==
-Crystal structure of the SarS protein from Staphylococcus aureus., Li R, Manna AC, Dai S, Cheung AL, Zhang G, J Bacteriol. 2003 Jul;185(14):4219-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12837797 12837797]
-[[Category: Single protein]]
 [[Category: Staphylococcus aureus]]
-[[Category: Cheung, A L.]]
+[[Category: Cheung AL]]
-[[Category: Dai, S.]]
+[[Category: Dai S]]
-[[Category: Li, R.]]
+[[Category: Li R]]
-[[Category: Manna, A C.]]
+[[Category: Manna AC]]
-[[Category: Zhang, G.]]
+[[Category: Zhang G]]
-[[Category: winged-helix protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:54:53 2008''