1nw1: Difference between revisions

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-[[Image:1nw1.jpg|left|200px]]
- {{Structure
+==Crystal Structure of Choline Kinase==
-|PDB= 1nw1 |SIZE=350|CAPTION= <scene name='initialview01'>1nw1</scene>, resolution 2.02&Aring;
+<StructureSection load='1nw1' size='340' side='right'caption='[[1nw1]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
+<table><tr><td colspan='2'>[[1nw1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NW1 FirstGlance]. <br>
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Choline_kinase Choline kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.32 2.7.1.32] </span>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.02&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nw1 OCA], [https://pdbe.org/1nw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nw1 RCSB], [https://www.ebi.ac.uk/pdbsum/1nw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nw1 ProSAT]</span></td></tr>
-|RELATEDENTRY=
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nw1 OCA], [http://www.ebi.ac.uk/pdbsum/1nw1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nw1 RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/CKA2_CAEEL CKA2_CAEEL] Catalyzes the first step in phosphatidylcholine biosynthesis. May contribute to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine but the activity is much higher with choline.<ref>PMID:12758145</ref> <ref>PMID:14960577</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nw/1nw1_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nw1 ConSurf].
+<div style="clear:both"></div>
-'''Crystal Structure of Choline Kinase'''
+==See Also==
+*[[Choline kinase 3D structures|Choline kinase 3D structures]]
+*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
-==Overview==
+== References ==
-Choline kinase catalyzes the ATP-dependent phosphorylation of choline, the first committed step in the CDP-choline pathway for the biosynthesis of phosphatidylcholine. The 2.0 A crystal structure of a choline kinase from C. elegans (CKA-2) reveals that the enzyme is a homodimeric protein with each monomer organized into a two-domain fold. The structure is remarkably similar to those of protein kinases and aminoglycoside phosphotransferases, despite no significant similarity in amino acid sequence. Comparisons to the structures of other kinases suggest that ATP binds to CKA-2 in a pocket formed by highly conserved and catalytically important residues. In addition, a choline binding site is proposed to be near the ATP binding pocket and formed by several structurally flexible loops.
+<references/>
+__TOC__
-==About this Structure==
+</StructureSection>
-NW1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NW1 OCA].
-==Reference==
-The crystal structure of choline kinase reveals a eukaryotic protein kinase fold., Peisach D, Gee P, Kent C, Xu Z, Structure. 2003 Jun;11(6):703-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12791258 12791258]
 [[Category: Caenorhabditis elegans]]
-[[Category: Choline kinase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Gee P]]
-[[Category: Gee, P.]]
+[[Category: Kent C]]
-[[Category: Kent, C.]]
+[[Category: Peisach D]]
-[[Category: Peisach, D.]]
+[[Category: Xu Z]]
-[[Category: Xu, Z.]]
-[[Category: choline kinase]]
-[[Category: phospholipid synthesis]]
-[[Category: protein kinase fold]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:36:23 2008''