1mkj: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /> <applet load="1mkj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mkj, resolution 2.70Å" /> '''Human Kinesin Motor...
 
No edit summary
 
(18 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1mkj.gif|left|200px]]<br />
<applet load="1mkj" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1mkj, resolution 2.70&Aring;" />
'''Human Kinesin Motor Domain With Docked Neck Linker'''<br />


==Overview==
==Human Kinesin Motor Domain With Docked Neck Linker==
Crystal structures of the molecular motor kinesin show conformational, variability in a structural element called the neck linker. Conformational, change in the neck linker, initiated by ATP exchange, is thought to drive, the movement of kinesin along the microtubule track. We use site-specific, EPR measurements to show that when microtubules are absent, the neck, linker exists in equilibrium between two structural states (disordered and, 'docked'). The active site nucleotide does not control the position taken, by the neck linker. However, we find that sulfate can specifically bind, near the nucleotide site and stabilize the docked neck linker, conformation, which we confirmed by solving a new crystal structure., Comparing the crystal structures of our construct with the docked or, undocked neck linker reveals how microtubule binding may activate the, nucleotide-sensing mechanism of kinesin, allowing neck linker transitions, to power motility.
<StructureSection load='1mkj' size='340' side='right'caption='[[1mkj]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1mkj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MKJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mkj OCA], [https://pdbe.org/1mkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mkj RCSB], [https://www.ebi.ac.uk/pdbsum/1mkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mkj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KINH_HUMAN KINH_HUMAN] Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mk/1mkj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mkj ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1MKJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, SO4 and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MKJ OCA].
*[[Kinesin 3D Structures|Kinesin 3D Structures]]
 
__TOC__
==Reference==
</StructureSection>
Two conformations in the human kinesin power stroke defined by X-ray crystallography and EPR spectroscopy., Sindelar CV, Budny MJ, Rice S, Naber N, Fletterick R, Cooke R, Nat Struct Biol. 2002 Nov;9(11):844-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12368902 12368902]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Budny, M.J.]]
[[Category: Budny MJ]]
[[Category: Cooke, R.]]
[[Category: Cooke R]]
[[Category: Fletterick, R.]]
[[Category: Fletterick R]]
[[Category: Naber, N.]]
[[Category: Naber N]]
[[Category: Rice, S.]]
[[Category: Rice S]]
[[Category: Sindelar, C.V.]]
[[Category: Sindelar CV]]
[[Category: ADP]]
[[Category: MG]]
[[Category: SO4]]
[[Category: atpase]]
[[Category: microtubule]]
[[Category: motor protein]]
[[Category: myosin]]
[[Category: neck linker]]
[[Category: relay helix]]
[[Category: switch ii]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:12:39 2007''

Latest revision as of 10:44, 14 February 2024

Human Kinesin Motor Domain With Docked Neck LinkerHuman Kinesin Motor Domain With Docked Neck Linker

Structural highlights

1mkj is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.7Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KINH_HUMAN Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1mkj, resolution 2.70Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1mkj&oldid=4051615"