1eqq: Difference between revisions

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New page: left|200px<br /><applet load="1eqq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eqq, resolution 3.2Å" /> '''SINGLE STRANDED DNA B...
 
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[[Image:1eqq.gif|left|200px]]<br /><applet load="1eqq" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1eqq, resolution 3.2&Aring;" />
'''SINGLE STRANDED DNA BINDING PROTEIN AND SSDNA COMPLEX'''<br />


==Overview==
==SINGLE STRANDED DNA BINDING PROTEIN AND SSDNA COMPLEX==
The single-stranded DNA (ssDNA) binding protein from Escherichia coli, (EcoSSB) plays a central role in DNA replication, recombination and, repair. The tertiary structure of EcoSSB was determined at 2.2 A, resolution. This is rather higher resolution than previously reported., Crystals were grown from the homogeneous intact protein but the EcoSSB, tetramer in the crystals contains truncated subunits lacking a part of the, C-terminal. The structure determined includes biologically important, flexible loops and C-terminal regions, and revealed the existence of, concavities. These concavities include the residues important for ssDNA, binding. An ssDNA can be fitted on the concavities and further stabilized, through interactions with the loops forming flexible lids. It seems likely, to play a central role in the binding of ssDNA.
<StructureSection load='1eqq' size='340' side='right'caption='[[1eqq]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1eqq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EQQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eqq OCA], [https://pdbe.org/1eqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eqq RCSB], [https://www.ebi.ac.uk/pdbsum/1eqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eqq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SSB_ECOLI SSB_ECOLI] This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eq/1eqq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eqq ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1EQQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EQQ OCA].
*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]]
 
*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
==Reference==
__TOC__
Roles of functional loops and the C-terminal segment of a single-stranded DNA binding protein elucidated by X-Ray structure analysis., Matsumoto T, Morimoto Y, Shibata N, Kinebuchi T, Shimamoto N, Tsukihara T, Yasuoka N, J Biochem (Tokyo). 2000 Feb;127(2):329-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10731701 10731701]
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Matsumoto, T.]]
[[Category: Matsumoto T]]
[[Category: Morimoto, Y.]]
[[Category: Morimoto Y]]
[[Category: Shibata, N.]]
[[Category: Shibata N]]
[[Category: Shimamoto, N.]]
[[Category: Shimamoto N]]
[[Category: Yasuoka, N.]]
[[Category: Yasuoka N]]
[[Category: beta barrel]]
[[Category: protein-dna complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:14:09 2007''

Latest revision as of 10:06, 7 February 2024

SINGLE STRANDED DNA BINDING PROTEIN AND SSDNA COMPLEXSINGLE STRANDED DNA BINDING PROTEIN AND SSDNA COMPLEX

Structural highlights

1eqq is a 6 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SSB_ECOLI This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1eqq, resolution 3.20Å

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