1eqq: Difference between revisions

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==SINGLE STRANDED DNA BINDING PROTEIN AND SSDNA COMPLEX==
==SINGLE STRANDED DNA BINDING PROTEIN AND SSDNA COMPLEX==
<StructureSection load='1eqq' size='340' side='right' caption='[[1eqq]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<StructureSection load='1eqq' size='340' side='right'caption='[[1eqq]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1eqq]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EQQ FirstGlance]. <br>
<table><tr><td colspan='2'>[[1eqq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EQQ FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1qvc|1qvc]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eqq OCA], [http://pdbe.org/1eqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eqq RCSB], [http://www.ebi.ac.uk/pdbsum/1eqq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1eqq ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eqq OCA], [https://pdbe.org/1eqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eqq RCSB], [https://www.ebi.ac.uk/pdbsum/1eqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eqq ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/SSB_ECOLI SSB_ECOLI]] This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair.  
[https://www.uniprot.org/uniprot/SSB_ECOLI SSB_ECOLI] This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eqq ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eqq ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The single-stranded DNA (ssDNA) binding protein from Escherichia coli (EcoSSB) plays a central role in DNA replication, recombination and repair. The tertiary structure of EcoSSB was determined at 2.2 A resolution. This is rather higher resolution than previously reported. Crystals were grown from the homogeneous intact protein but the EcoSSB tetramer in the crystals contains truncated subunits lacking a part of the C-terminal. The structure determined includes biologically important flexible loops and C-terminal regions, and revealed the existence of concavities. These concavities include the residues important for ssDNA binding. An ssDNA can be fitted on the concavities and further stabilized through interactions with the loops forming flexible lids. It seems likely to play a central role in the binding of ssDNA.


Roles of functional loops and the C-terminal segment of a single-stranded DNA binding protein elucidated by X-Ray structure analysis.,Matsumoto T, Morimoto Y, Shibata N, Kinebuchi T, Shimamoto N, Tsukihara T, Yasuoka N J Biochem. 2000 Feb;127(2):329-35. PMID:10731701<ref>PMID:10731701</ref>
==See Also==
 
*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
</div>
<div class="pdbe-citations 1eqq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus coli migula 1895]]
[[Category: Escherichia coli]]
[[Category: Matsumoto, T]]
[[Category: Large Structures]]
[[Category: Morimoto, Y]]
[[Category: Matsumoto T]]
[[Category: Shibata, N]]
[[Category: Morimoto Y]]
[[Category: Shimamoto, N]]
[[Category: Shibata N]]
[[Category: Yasuoka, N]]
[[Category: Shimamoto N]]
[[Category: Beta barrel]]
[[Category: Yasuoka N]]
[[Category: Protein-dna complex]]
[[Category: Replication-rna complex]]

Latest revision as of 10:06, 7 February 2024

SINGLE STRANDED DNA BINDING PROTEIN AND SSDNA COMPLEXSINGLE STRANDED DNA BINDING PROTEIN AND SSDNA COMPLEX

Structural highlights

1eqq is a 6 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SSB_ECOLI This protein is essential for replication of the chromosomes and its single-stranded DNA phages. It is also involved in DNA recombination and repair.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1eqq, resolution 3.20Å

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