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[[Image:1dtn.gif|left|200px]]<br />
<applet load="1dtn" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1dtn, resolution 2.1&Aring;" />
'''MANDELATE RACEMASE MUTANT D270N CO-CRYSTALLIZED WITH (S)-ATROLACTATE'''<br />


==Overview==
==MANDELATE RACEMASE MUTANT D270N CO-CRYSTALLIZED WITH (S)-ATROLACTATE==
In the high-resolution X-ray structure of mandelate racemase (MR) with the, competitive inhibitor (S)-atrolactate bound in the active site [Landro, J., A., Gerlt, J. A., Kozarich, J. W., Koo, C. W., Shah, V. J., Kenyon, G. L., Neidhart, D. J., Fujita, J., &amp; Petsko, G. A. (1994) Biochemistry 33, 635-643], the carboxylic acid group of Glu 317 is hydrogen-bonded to the, carboxylate group of the bound inhibitor. This geometry suggests that the, carboxylic acid functional group of Glu 317 participates as a general acid, catalyst in the concerted general acid-general base catalyzed formation of, a stabilized enolic tautomer of mandelic acid as a reaction intermediate., To test this hypothesis, the E317Q mutant of MR was constructed and, subjected to high-resolution X-ray structural analysis ... [[http://ispc.weizmann.ac.il/pmbin/getpm?7893689 (full description)]]
<StructureSection load='1dtn' size='340' side='right'caption='[[1dtn]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1dtn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DTN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APG:ATROLACTIC+ACID+(2-PHENYL-LACTIC+ACID)'>APG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dtn OCA], [https://pdbe.org/1dtn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dtn RCSB], [https://www.ebi.ac.uk/pdbsum/1dtn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dtn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MANR_PSEPU MANR_PSEPU]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dt/1dtn_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dtn ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1DTN is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with MG and APG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Isomerase Isomerase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.2 5.1.2.2]]. Structure known Active Sites: ACT, CAR and MTL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DTN OCA]].
*[[Mandelate racemase|Mandelate racemase]]
 
__TOC__
==Reference==
</StructureSection>
Mechanism of the reaction catalyzed by mandelate racemase: importance of electrophilic catalysis by glutamic acid 317., Mitra B, Kallarakal AT, Kozarich JW, Gerlt JA, Clifton JG, Petsko GA, Kenyon GL, Biochemistry. 1995 Mar 7;34(9):2777-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7893689 7893689]
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Single protein]]
[[Category: Clifton JG]]
[[Category: Clifton, J.G.]]
[[Category: Petsko GA]]
[[Category: Petsko, G.A.]]
[[Category: APG]]
[[Category: MG]]
[[Category: isomerase]]
[[Category: mandelate pathway]]
[[Category: racemase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:32:30 2007''

Latest revision as of 09:59, 7 February 2024

MANDELATE RACEMASE MUTANT D270N CO-CRYSTALLIZED WITH (S)-ATROLACTATEMANDELATE RACEMASE MUTANT D270N CO-CRYSTALLIZED WITH (S)-ATROLACTATE

Structural highlights

1dtn is a 1 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MANR_PSEPU

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1dtn, resolution 2.10Å

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