Mandelate racemase

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Function

Mandelate racemase (MAR) catalyzes the interconversion of enantiomers of mandelate (S to R). MAR is Mg-dependent. MAR can racemize other alpha-hydroxy carbonyl compounds. MAR is involved in aromatic acids catabolism[1].

Structural highlights

The biological assembly of mandelate racemase from Pseudomonas putida is . The [2]. Water molecule are shown as red sphere.

Structure of mandelate racemase complex with reaction intermediate phenyl-lactate and Mg+2 ion (green) (PDB entry 1mdr)

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3D structures of mandelate racemase3D structures of mandelate racemase

Updated on 28-October-2021

ReferencesReferences

  1. Powers VM, Koo CW, Kenyon GL, Gerlt JA, Kozarich JW. Mechanism of the reaction catalyzed by mandelate racemase. 1. Chemical and kinetic evidence for a two-base mechanism. Biochemistry. 1991 Sep 24;30(38):9255-63. PMID:1892833
  2. Landro JA, Gerlt JA, Kozarich JW, Koo CW, Shah VJ, Kenyon GL, Neidhart DJ, Fujita S, Petsko GA. The role of lysine 166 in the mechanism of mandelate racemase from Pseudomonas putida: mechanistic and crystallographic evidence for stereospecific alkylation by (R)-alpha-phenylglycidate. Biochemistry. 1994 Jan 25;33(3):635-43. PMID:8292591

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