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[[Image:1bg2.gif|left|200px]]<br /><applet load="1bg2" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1bg2, resolution 1.8&Aring;" />
'''HUMAN UBIQUITOUS KINESIN MOTOR DOMAIN'''<br />


==Overview==
==HUMAN UBIQUITOUS KINESIN MOTOR DOMAIN==
Kinesin is the founding member of a superfamily of microtubule based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. It has two identical approximately 960-amino-acid chains containing an amino-terminal globular motor domain, a central alpha-helical region that enables dimer formation through a coiled-coil, and a carboxy-terminal tail domain that binds light chains and possibly an organelle receptor. The kinesin motor domain of approximately 340 amino acids, which can produce movement in vitro, is much smaller than that of myosin (approximately 850 amino acids) and dynein (1,000 amino acids), and is the smallest known molecular motor. Here, we report the crystal structure of the human kinesin motor domain with bound ADP determined to 1.8-A resolution by X-ray crystallography. The motor consists primarily of a single alpha/beta arrowhead-shaped domain with dimensions of 70 x 45 x 45 A. Unexpectedly, it has a striking structural similarity to the core of the catalytic domain of the actin-based motor myosin. Although kinesin and myosin have virtually no amino-acid sequence++ identity, and exhibit distinct enzymatic and motile properties, our results suggest that these two classes of mechanochemical enzymes evolved from a common ancestor and share a similar force-generating strategy.
<StructureSection load='1bg2' size='340' side='right'caption='[[1bg2]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1bg2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. The April 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Kinesin''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_4 10.2210/rcsb_pdb/mom_2005_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BG2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bg2 OCA], [https://pdbe.org/1bg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bg2 RCSB], [https://www.ebi.ac.uk/pdbsum/1bg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bg2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KINH_HUMAN KINH_HUMAN] Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bg/1bg2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bg2 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1BG2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1BG2 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb64_1.html Kinesin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BG2 OCA].
*[[Kinesin 3D Structures|Kinesin 3D Structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of the kinesin motor domain reveals a structural similarity to myosin., Kull FJ, Sablin EP, Lau R, Fletterick RJ, Vale RD, Nature. 1996 Apr 11;380(6574):550-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8606779 8606779]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Kinesin]]
[[Category: Kinesin]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Fletterick, R J.]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Kull, F J.]]
[[Category: Fletterick RJ]]
[[Category: Lau, R.]]
[[Category: Kull FJ]]
[[Category: Sablin, E P.]]
[[Category: Lau R]]
[[Category: Vale, R D.]]
[[Category: Sablin EP]]
[[Category: ACT]]
[[Category: Vale RD]]
[[Category: ADP]]
[[Category: MG]]
[[Category: atpase]]
[[Category: microtubule associated]]
[[Category: motor protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:54:52 2008''

Latest revision as of 09:36, 7 February 2024

HUMAN UBIQUITOUS KINESIN MOTOR DOMAINHUMAN UBIQUITOUS KINESIN MOTOR DOMAIN

Structural highlights

1bg2 is a 1 chain structure with sequence from Homo sapiens. The April 2005 RCSB PDB Molecule of the Month feature on Kinesin by David S. Goodsell is 10.2210/rcsb_pdb/mom_2005_4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KINH_HUMAN Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1bg2, resolution 1.80Å

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OCA
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