5by4: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 5by4 is ON HOLD
+==Structure and function of the Escherichia coli Tol-Pal stator protein TolR==
+<StructureSection load='5by4' size='340' side='right'caption='[[5by4]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5by4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BY4 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.702&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5by4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5by4 OCA], [https://pdbe.org/5by4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5by4 RCSB], [https://www.ebi.ac.uk/pdbsum/5by4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5by4 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TOLR_ECOLI TOLR_ECOLI] Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+TolR is a 15-kDa inner membrane protein subunit of the Tol-Pal complex in Gram- negative bacteria the function of which is poorly understood. Tol-Pal is recruited to cell division sites where it is involved in maintaining the integrity of the outer membrane. TolR is related to MotB, the peptidoglycan (PG)-binding stator protein from the flagellum, suggesting it might serve a similar role in Tol-Pal. The only structure thus far reported for TolR is of the periplasmic domain from Haemophilus influenzae in which N- and C-terminal residues had been deleted (TolR62-133, E. coli numbering). H. influenzae TolR62-133 is a symmetrical dimer with a large deep cleft at the dimer interface. Here, we present the 1.7 A crystal structure of the intact periplasmic domain of Escherichia coli TolR (TolR36-142). E. coli TolR36-142 is also dimeric but the architecture of the dimer is radically different to that of TolR62-133 due to intertwining of its N- and C-termini. TolR monomers are rotated ~180 degrees relative to each other as a result of this strand-swapping, obliterating the putative PG-binding groove seen in TolR62-133. We found that removal of the strand swapped regions (TolR60-133) exposes cryptic PG binding activity that is absent in the full-length domain. We conclude that to function as a stator in the Tol-Pal complex dimeric TolR must undergo large- scale structural remodelling reminiscent of that proposed for MotB where N- and C-terminal sequences unfold in order for the protein to both reach and bind the PG layer ~90 A away from the inner membrane.
-Authors: Wojdyla, J.A., Kaminska, R., Kleanthous, C.
+Structure and Function of the Escherichia coli Tol-Pal Stator Protein TolR.,Wojdyla JA, Cutts E, Kaminska R, Papadakos G, Hopper JT, Stansfeld PJ, Staunton D, Robinson CV, Kleanthous C J Biol Chem. 2015 Sep 9. pii: jbc.M115.671586. PMID:26354441<ref>PMID:26354441</ref>
-Description: Structure and function of the Escherichia coli Tol-Pal stator protein TolR
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Wojdyla, J.A]]
+<div class="pdbe-citations 5by4" style="background-color:#fffaf0;"></div>
-[[Category: Kleanthous, C]]
-[[Category: Kaminska, R]]
+==See Also==
+*[[TolR|TolR]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli]]
+[[Category: Large Structures]]
+[[Category: Kaminska R]]
+[[Category: Kleanthous C]]
+[[Category: Wojdyla JA]]