1ull: Difference between revisions

<StructureSection load='1ull' size='340' side='right' caption='[[1ull]], [[NMR_Ensembles_of_Models | 7 NMR models]]' scene=''>

<table><tr><td colspan='2'>[[1ull]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ULL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ULL FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ull FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ull OCA], [http://pdbe.org/1ull PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ull RCSB], [http://www.ebi.ac.uk/pdbsum/1ull PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ull ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/REV_HV1W2 REV_HV1W2]] Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rev itself is translated from a fully spliced mRNA that readily exits the nucleus. Rev's nuclear localization signal (NLS) binds directly to KPNB1/Importin beta-1 without previous binding to KPNA1/Importin alpha-1. KPNB1 binds to the GDP bound form of RAN (Ran-GDP) and targets Rev to the nucleus. In the nucleus, the conversion from Ran-GDP to Ran-GTP dissociates Rev from KPNB1 and allows Rev's binding to the RRE in viral pre-mRNAs. Rev multimerization on the RRE via cooperative assembly exposes its nuclear export signal (NES) to the surface. Rev can then form a complex with XPO1/CRM1 and Ran-GTP, leading to nuclear export of the complex. Conversion from Ran-GTP to Ran-GDP mediates dissociation of the Rev/RRE/XPO1/RAN complex, so that Rev can return to the nucleus for a subsequent round of export. Beside KPNB1, also seems to interact with TNPO1/Transportin-1, RANBP5/IPO5 and IPO7/RANBP7 for nuclear import. The nucleoporin-like HRB/RIP is an essential cofactor that probably indirectly interacts with Rev to release HIV RNAs from the perinuclear region to the cytoplasm (By similarity).  

[[Category: Ellington, A D]]

[[Category: Gorin, A]]

[[Category: Patel, D J]]

[[Category: Ye, X]]

[[Category: Adaptive binding of peptide and rna in complex]]

[[Category: Alpha-helix in widened rna major groove]]

[[Category: Viral protein-rna complex]]