7c9p: Difference between revisions
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==== | ==Crystal structure of rice histone-fold dimer GHD8/OsNF-YC2== | ||
<StructureSection load='7c9p' size='340' side='right'caption='[[7c9p]]' scene=''> | <StructureSection load='7c9p' size='340' side='right'caption='[[7c9p]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[7c9p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C9P FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c9p OCA], [https://pdbe.org/7c9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c9p RCSB], [https://www.ebi.ac.uk/pdbsum/7c9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c9p ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/HD5_ORYSJ HD5_ORYSJ] Probable transcription factor involved in the regulation of flowering time under long day (LD) conditions. Functions as repressor of flowering, independently of HD1 and GHD7. Controls flowering time by negatively regulating the expression of EHD1 and HD3A (PubMed:20566706, PubMed:21148627). Regulates plant height by promoting cell elongation in the internodes (PubMed:20566706). Component of the NF-Y/HAP transcription factor complex (By similarity).<ref>PMID:20566706</ref> <ref>PMID:21148627</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CCT (CONSTANS, CONSTANS-LIKE and TOC1) domain-containing proteins are a large family unique to plants. They transcriptionally regulate the processes related to photoperiodic flowering, circadian rhythms, vernalization, and other related functions. CO (CONSTANS) and HD1 (HEADING DATE 1), through the CCT domain, coordinate with the NF-YB/YC dimer to specifically target a conserved 'CCACA' motif within the downstream promotor. However, the mechanism underlying the DNA recognition by CCT domain remains unclear. Here we determined the crystal structures of the rice NF-YB/YC dimer and the florigen Hd3a-bound HD1CCT/NF-YB/YC trimer with resolutions of 2.0 A and 2.55 A, respectively. The CCT domain of HD1 displays an elongated structure containing two (alpha)-helices and two loops, tethering the Hd3a to the NF-YB/YC dimer. Helix (alpha)2 and loop 2 are anchored into the minor groove of the 'CCACA' motif, which determines the specific base recognition. Our structures reveal the interaction mechanism among the CCT domain, NF-YB/YC dimer, and the target DNA. These results not only provide new insights into the network between the CCT proteins and NF-Y subunits, but also offer references for improving productivity and global adaptability of crops by manipulating florigen expression. | |||
Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering.,Shen C, Liu H, Guan Z, Junjie Y, Ting Z, Yan W, Wu C, Zhang Q, Yin P, Xing Y Plant Cell. 2020 Aug 25. pii: tpc.20.00067. doi: 10.1105/tpc.20.00067. PMID:32843433<ref>PMID:32843433</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7c9p" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Nuclear transcription factor Y|Nuclear transcription factor Y]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Z | [[Category: Oryza sativa Japonica Group]] | ||
[[Category: Guan Z]] | |||
[[Category: Liu H]] | |||
[[Category: Shen C]] | |||
[[Category: Xing Y]] | |||
[[Category: Yin P]] | |||