7c9p

Crystal structure of rice histone-fold dimer GHD8/OsNF-YC2Crystal structure of rice histone-fold dimer GHD8/OsNF-YC2

Structural highlights

7c9p is a 4 chain structure with sequence from Oryza sativa Japonica Group. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HD5_ORYSJ Probable transcription factor involved in the regulation of flowering time under long day (LD) conditions. Functions as repressor of flowering, independently of HD1 and GHD7. Controls flowering time by negatively regulating the expression of EHD1 and HD3A (PubMed:20566706, PubMed:21148627). Regulates plant height by promoting cell elongation in the internodes (PubMed:20566706). Component of the NF-Y/HAP transcription factor complex (By similarity).^[1] ^[2]

Publication Abstract from PubMed

CCT (CONSTANS, CONSTANS-LIKE and TOC1) domain-containing proteins are a large family unique to plants. They transcriptionally regulate the processes related to photoperiodic flowering, circadian rhythms, vernalization, and other related functions. CO (CONSTANS) and HD1 (HEADING DATE 1), through the CCT domain, coordinate with the NF-YB/YC dimer to specifically target a conserved 'CCACA' motif within the downstream promotor. However, the mechanism underlying the DNA recognition by CCT domain remains unclear. Here we determined the crystal structures of the rice NF-YB/YC dimer and the florigen Hd3a-bound HD1CCT/NF-YB/YC trimer with resolutions of 2.0 A and 2.55 A, respectively. The CCT domain of HD1 displays an elongated structure containing two (alpha)-helices and two loops, tethering the Hd3a to the NF-YB/YC dimer. Helix (alpha)2 and loop 2 are anchored into the minor groove of the 'CCACA' motif, which determines the specific base recognition. Our structures reveal the interaction mechanism among the CCT domain, NF-YB/YC dimer, and the target DNA. These results not only provide new insights into the network between the CCT proteins and NF-Y subunits, but also offer references for improving productivity and global adaptability of crops by manipulating florigen expression.

Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering.,Shen C, Liu H, Guan Z, Junjie Y, Ting Z, Yan W, Wu C, Zhang Q, Yin P, Xing Y Plant Cell. 2020 Aug 25. pii: tpc.20.00067. doi: 10.1105/tpc.20.00067. PMID:32843433^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wei X, Xu J, Guo H, Jiang L, Chen S, Yu C, Zhou Z, Hu P, Zhai H, Wan J. DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously. Plant Physiol. 2010 Aug;153(4):1747-58. PMID:20566706 doi:10.1104/pp.110.156943
↑ Yan WH, Wang P, Chen HX, Zhou HJ, Li QP, Wang CR, Ding ZH, Zhang YS, Yu SB, Xing YZ, Zhang QF. A major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice. Mol Plant. 2011 Mar;4(2):319-30. PMID:21148627 doi:10.1093/mp/ssq070
↑ Shen C, Liu H, Guan Z, Yan J, Zheng T, Yan W, Wu C, Zhang Q, Yin P, Xing Y. Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering. Plant Cell. 2020 Nov;32(11):3469-3484. PMID:32843433 doi:10.1105/tpc.20.00067

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OCA

[1] Wei X, Xu J, Guo H, Jiang L, Chen S, Yu C, Zhou Z, Hu P, Zhai H, Wan J. DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously. Plant Physiol. 2010 Aug;153(4):1747-58. PMID:20566706 doi:10.1104/pp.110.156943

[2] Yan WH, Wang P, Chen HX, Zhou HJ, Li QP, Wang CR, Ding ZH, Zhang YS, Yu SB, Xing YZ, Zhang QF. A major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice. Mol Plant. 2011 Mar;4(2):319-30. PMID:21148627 doi:10.1093/mp/ssq070

[3] Shen C, Liu H, Guan Z, Yan J, Zheng T, Yan W, Wu C, Zhang Q, Yin P, Xing Y. Structural Insight into DNA Recognition by CCT/NF-YB/YC Complexes in Plant Photoperiodic Flowering. Plant Cell. 2020 Nov;32(11):3469-3484. PMID:32843433 doi:10.1105/tpc.20.00067

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