6l9c: Difference between revisions
New page: '''Unreleased structure''' The entry 6l9c is ON HOLD Authors: Murakawa, T., Kurihara, K., Shoji, M., Shibazaki, C., Sunami, T., Tamada, T., Yano, N., Yamada, T., Kusaka, K., Suzuki, M.,... |
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==Neutron structure of copper amine oxidase from Arthrobacter glibiformis at pD 7.4== | |||
<StructureSection load='6l9c' size='340' side='right'caption='[[6l9c]], [[Resolution|resolution]] 1.14Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6l9c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L9C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L9C FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Neutron Diffraction , X-ray diffraction, [[Resolution|Resolution]] 1.14Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASA:ASPARTIC+ALDEHYDE'>ASA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=E9C:(2S)-2-azanyl-3-[3,4,6-tris(oxidanylidene)cyclohexen-1-yl]propanoic+acid'>E9C</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TPQ:5-(2-CARBOXY-2-AMINOETHYL)-2-HYDROXY-1,4-BENZOQUINONE'>TPQ</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l9c OCA], [https://pdbe.org/6l9c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l9c RCSB], [https://www.ebi.ac.uk/pdbsum/6l9c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l9c ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/PAOX_ARTGO PAOX_ARTGO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Recent advances in neutron crystallographic studies have provided structural bases for quantum behaviors of protons observed in enzymatic reactions. Thus, we resolved the neutron crystal structure of a bacterial copper (Cu) amine oxidase (CAO), which contains a prosthetic Cu ion and a protein-derived redox cofactor, topa quinone (TPQ). We solved hitherto unknown structures of the active site, including a keto/enolate equilibrium of the cofactor with a nonplanar quinone ring, unusual proton sharing between the cofactor and the catalytic base, and metal-induced deprotonation of a histidine residue that coordinates to the Cu. Our findings show a refined active-site structure that gives detailed information on the protonation state of dissociable groups, such as the quinone cofactor, which are critical for catalytic reactions. | |||
Neutron crystallography of copper amine oxidase reveals keto/enolate interconversion of the quinone cofactor and unusual proton sharing.,Murakawa T, Kurihara K, Shoji M, Shibazaki C, Sunami T, Tamada T, Yano N, Yamada T, Kusaka K, Suzuki M, Shigeta Y, Kuroki R, Hayashi H, Yano T, Tanizawa K, Adachi M, Okajima T Proc Natl Acad Sci U S A. 2020 May 5. pii: 1922538117. doi:, 10.1073/pnas.1922538117. PMID:32371483<ref>PMID:32371483</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6l9c" style="background-color:#fffaf0;"></div> | ||
[[Category: Hayashi | |||
[[Category: | ==See Also== | ||
[[Category: Kuroki | *[[Copper amine oxidase 3D structures|Copper amine oxidase 3D structures]] | ||
[[Category: Kusaka | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Arthrobacter globiformis]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Adachi M]] | ||
[[Category: Suzuki | [[Category: Hayashi H]] | ||
[[Category: | [[Category: Kurihara K]] | ||
[[Category: | [[Category: Kuroki R]] | ||
[[Category: | [[Category: Kusaka K]] | ||
[[Category: | [[Category: Murakawa T]] | ||
[[Category: | [[Category: Okajima T]] | ||
[[Category: Shibazaki C]] | |||
[[Category: Shigeta Y]] | |||
[[Category: Shoji M]] | |||
[[Category: Sunami T]] | |||
[[Category: Suzuki M]] | |||
[[Category: Tamada T]] | |||
[[Category: Tanizawa K]] | |||
[[Category: Yamada T]] | |||
[[Category: Yano N]] | |||
[[Category: Yano Y]] | |||