6l9c
Neutron structure of copper amine oxidase from Arthrobacter glibiformis at pD 7.4Neutron structure of copper amine oxidase from Arthrobacter glibiformis at pD 7.4
Structural highlights
FunctionPublication Abstract from PubMedRecent advances in neutron crystallographic studies have provided structural bases for quantum behaviors of protons observed in enzymatic reactions. Thus, we resolved the neutron crystal structure of a bacterial copper (Cu) amine oxidase (CAO), which contains a prosthetic Cu ion and a protein-derived redox cofactor, topa quinone (TPQ). We solved hitherto unknown structures of the active site, including a keto/enolate equilibrium of the cofactor with a nonplanar quinone ring, unusual proton sharing between the cofactor and the catalytic base, and metal-induced deprotonation of a histidine residue that coordinates to the Cu. Our findings show a refined active-site structure that gives detailed information on the protonation state of dissociable groups, such as the quinone cofactor, which are critical for catalytic reactions. Neutron crystallography of copper amine oxidase reveals keto/enolate interconversion of the quinone cofactor and unusual proton sharing.,Murakawa T, Kurihara K, Shoji M, Shibazaki C, Sunami T, Tamada T, Yano N, Yamada T, Kusaka K, Suzuki M, Shigeta Y, Kuroki R, Hayashi H, Yano T, Tanizawa K, Adachi M, Okajima T Proc Natl Acad Sci U S A. 2020 May 5. pii: 1922538117. doi:, 10.1073/pnas.1922538117. PMID:32371483[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||