8pfb: Difference between revisions
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==Structure of a heteropolymeric type 4 pilus from a monoderm bacterium== | |||
<StructureSection load='8pfb' size='340' side='right'caption='[[8pfb]], [[Resolution|resolution]] 3.67Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8pfb]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sanguinis Streptococcus sanguinis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PFB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PFB FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.67Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pfb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pfb OCA], [https://pdbe.org/8pfb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pfb RCSB], [https://www.ebi.ac.uk/pdbsum/8pfb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pfb ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0B7GU52_STRSA A0A0B7GU52_STRSA] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Type 4 pili (T4P) are important virulence factors, which belong to a superfamily of nanomachines ubiquitous in prokaryotes, called type 4 filaments (T4F). T4F are defined as helical polymers of type 4 pilins. Recent advances in cryo-electron microscopy (cryo-EM) led to structures of several T4F, revealing that the long N-terminal alpha-helix (alpha1) - the trademark of pilins - packs in the centre of the filaments to form a hydrophobic core. In diderm bacteria - all available bacterial T4F structures are from diderm species - a portion of alpha1 is melted (unfolded). Here we report that this architecture is conserved in phylogenetically distant monoderm species by determining the structure of Streptococcus sanguinis T4P. Our 3.7 A resolution cryo-EM structure of S. sanguinis heteropolymeric T4P and the resulting full atomic model including all minor pilins highlight universal features of bacterial T4F and have widespread implications in understanding T4F biology. | |||
Structure of a heteropolymeric type 4 pilus from a monoderm bacterium.,Anger R, Pieulle L, Shahin M, Valette O, Le Guenno H, Kosta A, Pelicic V, Fronzes R Nat Commun. 2023 Nov 6;14(1):7143. doi: 10.1038/s41467-023-42872-5. PMID:37932265<ref>PMID:37932265</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8pfb" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Shahin | [[Category: Streptococcus sanguinis]] | ||
[[Category: | [[Category: Anger R]] | ||
[[Category: Fronzes R]] | |||
[[Category: Kosta A]] | |||
[[Category: Le Guenno H]] | |||
[[Category: Pelicic V]] | |||
[[Category: Pieulle L]] | |||
[[Category: Shahin M]] | |||
[[Category: Valette O]] | |||
Latest revision as of 10:30, 22 November 2023
Structure of a heteropolymeric type 4 pilus from a monoderm bacteriumStructure of a heteropolymeric type 4 pilus from a monoderm bacterium
Structural highlights
FunctionPublication Abstract from PubMedType 4 pili (T4P) are important virulence factors, which belong to a superfamily of nanomachines ubiquitous in prokaryotes, called type 4 filaments (T4F). T4F are defined as helical polymers of type 4 pilins. Recent advances in cryo-electron microscopy (cryo-EM) led to structures of several T4F, revealing that the long N-terminal alpha-helix (alpha1) - the trademark of pilins - packs in the centre of the filaments to form a hydrophobic core. In diderm bacteria - all available bacterial T4F structures are from diderm species - a portion of alpha1 is melted (unfolded). Here we report that this architecture is conserved in phylogenetically distant monoderm species by determining the structure of Streptococcus sanguinis T4P. Our 3.7 A resolution cryo-EM structure of S. sanguinis heteropolymeric T4P and the resulting full atomic model including all minor pilins highlight universal features of bacterial T4F and have widespread implications in understanding T4F biology. Structure of a heteropolymeric type 4 pilus from a monoderm bacterium.,Anger R, Pieulle L, Shahin M, Valette O, Le Guenno H, Kosta A, Pelicic V, Fronzes R Nat Commun. 2023 Nov 6;14(1):7143. doi: 10.1038/s41467-023-42872-5. PMID:37932265[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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