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==Bacteriophytochrome activated diguanylyl cyclase from Idiomarina species A28L== | ==Bacteriophytochrome activated diguanylyl cyclase from Idiomarina species A28L== | ||
<StructureSection load='5llw' size='340' side='right' caption='[[5llw]], [[Resolution|resolution]] 3.00Å' scene=''> | <StructureSection load='5llw' size='340' side='right'caption='[[5llw]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5llw]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LLW OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5llw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Idiomarina_sp._A28L Idiomarina sp. A28L]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LLW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LLW FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LBV:3-[2-[(Z)-[3-(2-CARBOXYETHYL)-5-[(Z)-(4-ETHENYL-3-METHYL-5-OXIDANYLIDENE-PYRROL-2-YLIDENE)METHYL]-4-METHYL-PYRROL-1-IUM-2-YLIDENE]METHYL]-5-[(Z)-[(3E)-3-ETHYLIDENE-4-METHYL-5-OXIDANYLIDENE-PYRROLIDIN-2-YLIDENE]METHYL]-4-METHYL-1H-PYRROL-3-YL]PROPANOIC+ACID'>LBV</scene> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LBV:3-[2-[(Z)-[3-(2-CARBOXYETHYL)-5-[(Z)-(4-ETHENYL-3-METHYL-5-OXIDANYLIDENE-PYRROL-2-YLIDENE)METHYL]-4-METHYL-PYRROL-1-IUM-2-YLIDENE]METHYL]-5-[(Z)-[(3E)-3-ETHYLIDENE-4-METHYL-5-OXIDANYLIDENE-PYRROLIDIN-2-YLIDENE]METHYL]-4-METHYL-1H-PYRROL-3-YL]PROPANOIC+ACID'>LBV</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5llw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5llw OCA], [https://pdbe.org/5llw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5llw RCSB], [https://www.ebi.ac.uk/pdbsum/5llw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5llw ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/F7RW09_9GAMM F7RW09_9GAMM] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nature has evolved an astonishingly modular architecture of covalently linked protein domains with diverse functionalities to enable complex cellular networks that are critical for cell survival. The coupling of sensory modules with enzymatic effectors allows direct allosteric regulation of cellular signaling molecules in response to diverse stimuli. We present molecular details of red light-sensing bacteriophytochromes linked to cyclic dimeric guanosine monophosphate-producing diguanylyl cyclases. Elucidation of the first crystal structure of a full-length phytochrome with its enzymatic effector, in combination with the characterization of light-induced changes in conformational dynamics, reveals how allosteric light regulation is fine-tuned by the architecture and composition of the coiled-coil sensor-effector linker and also the central helical spine. We anticipate that consideration of molecular principles of sensor-effector coupling, going beyond the length of the characteristic linker, and the appreciation of dynamically driven allostery will open up new directions for the design of novel red light-regulated optogenetic tools. | |||
Long-range allosteric signaling in red light-regulated diguanylyl cyclases.,Gourinchas G, Etzl S, Gobl C, Vide U, Madl T, Winkler A Sci Adv. 2017 Mar 3;3(3):e1602498. doi: 10.1126/sciadv.1602498. eCollection 2017 , Mar. PMID:28275738<ref>PMID:28275738</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5llw" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Diguanylate cyclase|Diguanylate cyclase]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Idiomarina sp. A28L]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Gourinchas G]] | ||
[[Category: | [[Category: Winkler A]] | ||
Latest revision as of 21:33, 18 October 2023
Bacteriophytochrome activated diguanylyl cyclase from Idiomarina species A28LBacteriophytochrome activated diguanylyl cyclase from Idiomarina species A28L
Structural highlights
FunctionPublication Abstract from PubMedNature has evolved an astonishingly modular architecture of covalently linked protein domains with diverse functionalities to enable complex cellular networks that are critical for cell survival. The coupling of sensory modules with enzymatic effectors allows direct allosteric regulation of cellular signaling molecules in response to diverse stimuli. We present molecular details of red light-sensing bacteriophytochromes linked to cyclic dimeric guanosine monophosphate-producing diguanylyl cyclases. Elucidation of the first crystal structure of a full-length phytochrome with its enzymatic effector, in combination with the characterization of light-induced changes in conformational dynamics, reveals how allosteric light regulation is fine-tuned by the architecture and composition of the coiled-coil sensor-effector linker and also the central helical spine. We anticipate that consideration of molecular principles of sensor-effector coupling, going beyond the length of the characteristic linker, and the appreciation of dynamically driven allostery will open up new directions for the design of novel red light-regulated optogenetic tools. Long-range allosteric signaling in red light-regulated diguanylyl cyclases.,Gourinchas G, Etzl S, Gobl C, Vide U, Madl T, Winkler A Sci Adv. 2017 Mar 3;3(3):e1602498. doi: 10.1126/sciadv.1602498. eCollection 2017 , Mar. PMID:28275738[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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