6xgx: Difference between revisions
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==== | ==ISCth4 transposase, strand transfer complex 1, STC1== | ||
<StructureSection load='6xgx' size='340' side='right'caption='[[6xgx]]' scene=''> | <StructureSection load='6xgx' size='340' side='right'caption='[[6xgx]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6xgx]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus_ATCC_27405 Acetivibrio thermocellus ATCC 27405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XGX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XGX FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xgx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xgx OCA], [https://pdbe.org/6xgx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xgx RCSB], [https://www.ebi.ac.uk/pdbsum/6xgx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xgx ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A3DBR0_ACET2 A3DBR0_ACET2] Required for the transposition of the insertion element.[ARBA:ARBA00002190][RuleBase:RU365089] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Copy-out/paste-in transposition is a major bacterial DNA mobility pathway. It contributes significantly to the emergence of antibiotic resistance, often by upregulating expression of downstream genes upon integration. Unlike other transposition pathways, it requires both asymmetric and symmetric strand transfer steps. Here, we report the first structural study of a copy-out/paste-in transposase and demonstrate its ability to catalyze all pathway steps in vitro. X-ray structures of ISCth4 transposase, a member of the IS256 family of insertion sequences, bound to DNA substrates corresponding to three sequential steps in the reaction reveal an unusual asymmetric dimeric transpososome. During transposition, an array of N-terminal domains binds a single transposon end while the catalytic domain moves to accommodate the varying substrates. These conformational changes control the path of DNA flanking the transposon end and the generation of DNA-binding sites. Our results explain the asymmetric outcome of the initial strand transfer and show how DNA binding is modulated by the asymmetric transposase to allow the capture of a second transposon end and to integrate a circular intermediate. | |||
Structures of ISCth4 transpososomes reveal the role of asymmetry in copy-out/paste-in DNA transposition.,Kosek D, Hickman AB, Ghirlando R, He S, Dyda F EMBO J. 2020 Oct 2:e105666. doi: 10.15252/embj.2020105666. PMID:33006208<ref>PMID:33006208</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6xgx" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Transposase 3D structures|Transposase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Acetivibrio thermocellus ATCC 27405]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Dyda F]] | ||
[[Category: Kosek D]] | |||