6xgx

ISCth4 transposase, strand transfer complex 1, STC1ISCth4 transposase, strand transfer complex 1, STC1

Structural highlights 6xgx is a 5 chain structure with sequence from Acetivibrio thermocellus ATCC 27405. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.5Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A3DBR0_ACET2 Required for the transposition of the insertion element.[ARBA:ARBA00002190][RuleBase:RU365089] Publication Abstract from PubMed Copy-out/paste-in transposition is a major bacterial DNA mobility pathway. It contributes significantly to the emergence of antibiotic resistance, often by upregulating expression of downstream genes upon integration. Unlike other transposition pathways, it requires both asymmetric and symmetric strand transfer steps. Here, we report the first structural study of a copy-out/paste-in transposase and demonstrate its ability to catalyze all pathway steps in vitro. X-ray structures of ISCth4 transposase, a member of the IS256 family of insertion sequences, bound to DNA substrates corresponding to three sequential steps in the reaction reveal an unusual asymmetric dimeric transpososome. During transposition, an array of N-terminal domains binds a single transposon end while the catalytic domain moves to accommodate the varying substrates. These conformational changes control the path of DNA flanking the transposon end and the generation of DNA-binding sites. Our results explain the asymmetric outcome of the initial strand transfer and show how DNA binding is modulated by the asymmetric transposase to allow the capture of a second transposon end and to integrate a circular intermediate. Structures of ISCth4 transpososomes reveal the role of asymmetry in copy-out/paste-in DNA transposition.,Kosek D, Hickman AB, Ghirlando R, He S, Dyda F EMBO J. 2020 Oct 2:e105666. doi: 10.15252/embj.2020105666. PMID:33006208 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Transposase 3D structures

spinqualitylabelsall models 6xgx, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image